Expression of Urinary Survivin as a New Tumor Marker for the Detection of Bladder Cancer.
- Author:
Jun Mo KIM
1
;
Kwang Woo LEE
;
Young Ho KIM
;
Sung Kyu PARK
;
Yoo Kyung LEE
;
Min Eui KIM
Author Information
1. Departments of Urology, Soonchunhyang University College of Medicine, Bucheon, Korea. mekim@schbc.ac.kr
- Publication Type:Original Article
- Keywords:
Survivin;
Tumor markers, biological;
Bladder cancer
- MeSH:
Adenocarcinoma;
Apoptosis;
Carcinoma, Small Cell;
Diagnostic Tests, Routine;
Enzyme-Linked Immunosorbent Assay;
Healthy Volunteers;
Humans;
Neoplasm Staging;
Prospective Studies;
Sensitivity and Specificity;
Biomarkers, Tumor;
Urinary Bladder Neoplasms*;
Urinary Bladder*;
Urologic Diseases
- From:Korean Journal of Urology
2004;45(12):1219-1223
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Survivin is a novel member of the inhibitor of apoptosis (IAP) gene family, which is over-expressed in many human tumors include bladder cancer. This prospective study was performed to investigate the clinical usefulness of a test for urinary survivin for the detection of bladder cancer. MATERIALS AND METHODS: From September 2002 and July 2003, voided urine samples were obtained from 86 healthy volunteers (group 1), from 25 patients having urologic diseases except bladder cancer (group 2), and from 48 patients with bladder cancer (group 3). One patient with small cell carcinoma, and three cases of adenocarcinoma of the bladder were included in group 3. Quantitative measurement of urinary survivin was analyzed using the DuoSet IC enzyme-linked immunosorbent assay (ELISA) kit and a microplate luminometer, DML2000. RESULTS: The mean urine level of survivin was 7.9pg/ml (1-23) in group 1, 6.0pg/ml (1-12) in group 2 and 63.3pg/ml (1-745) in group 3. Urinary survivin was over-expressed in group 3 more than group 1 and 2 (p<0.01). When we decided that the cut-off value of urinary survivin was to be past the mean 2SD in group 1, the normal upper limit was then 16.5pg/ml. The sensitivity and specificity for the detection of the bladder cancer were 77.1% and 86.8% respectively. The sensitivity and specificity of urinary survivin for urothelial cancer of the bladder were 85.0% and 96.0% respectively. The survivn expression for the high grade tumor was significantly higher than the low grade tumor (p=0.048), but there was no significant difference in the expression of urinary survivin according to the TNM staging (p=0.058). CONCLUSIONS: The results of our study showed that a diagnostic test with urinary survivin had a high sensitivity and specificity when the cut-off value of survivin was decided to be 16.5pg/ml. This suggests that urinary survivin might be an available tumor marker for the detection of bladder cancer.
