Effects of age and smoking on the expression of citrullinated histones in patients with periodontitis and the analysis of expression levels according to sample types: pilot study
10.14368/jdras.2024.40.4.234
- Author:
Gi-Tae PARK
1
;
Ju-Youn LEE
;
Ji-Young JOO
;
Dong Hyun SOHN
;
Hyun-Joo KIM
Author Information
1. Department of Periodontology, Dental and Life Science Institute, School of Dentistry, Pusan National University, Yangsan, Republic of Korea
- Publication Type:Original Article
- From:Journal of Dental Rehabilitation and Applied Science
2024;40(4):234-240
- CountryRepublic of Korea
- Language:English
-
Abstract:
Purpose:In chronic inflammatory diseases, neutrophils release NETs (neutrophil extracellular traps), web-like structures composedof DNA and citrullinated histones, to eliminate bacteria and regulate the inflammatory response. In periodontitis, NETosis acts asa crucial defense mechanism, characterized by the expression of citrullinated histones. This study aims to evaluate the impact ofage and smoking on the expression of citrullinated histones in patients with periodontitis and the feasibility of using saliva for their detection.
Materials and Methods:A total of 19 patients participated in the experiment. Among them, 15 were grouped accord-ing to smoking status and age, with 5 patients assigned to each group. After clinical and radiographic examinations, blood sampleswere collected from the subjects, and serum was separated. For 4 subjects, both blood and saliva samples were collected. Thelevels of citrullinated histones in the samples were quantified using an ELISA kit, and the results were compared and statisticallyanalyzed.
Results:The concentration of citrullinated histones was significantly higher in younger individuals (P < 0.05). Although the expression of citrullinated histones was higher in the smoking group compared to the non-smoking group, the difference was not statistically significant. In patients with periodontitis, the levels of citrullinated histones detected in saliva were comparable to those found in serum.
Conclusion:Age may be potential risk factors influencing the expression of citrullinated histones in patients with periodontitis. Additionally, saliva, which is easier to collect, could be used as a viable sample for detecting citrullinated histones in periodontitis patients.
