Mouse Orthotopic Lung Cancer Model Induced by PC14PE6.
- Author:
Zheng Yun CUI
1
;
Jin Seok AHN
;
Jee Yun LEE
;
Won Seog KIM
;
Ho Yeong LIM
;
Hyun Jung JEON
;
Soo Won SUH
;
Jin Hoon KIM
;
Won Ho KONG
;
Ji Min KANG
;
Do Hyun NAM
;
Keunchil PARK
Author Information
1. Medical Nano Element Development Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Orthotopic;
PC14PE6;
Lung cancer;
Animal model
- MeSH:
Adenocarcinoma;
Adrenal Glands;
Animals;
Brain;
Heterografts;
Humans;
Kidney;
Knee;
Liver;
Lung Neoplasms*;
Lung*;
Lymph Nodes;
Male;
Mice*;
Mice, Nude;
Models, Animal;
Neoplasm Metastasis;
Spleen;
Thorax
- From:Cancer Research and Treatment
2006;38(4):234-239
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: This study was undertaken to investigate in detail the xenograft mouse orthotopic lung cancer model induced by PC14PE6 adenocarcinoma cells. MATERIALS AND METHODS: Three cell doses (0.5x10(6); 1x10(6); 2x10(6)) of PC14PE6 cells were injected into the lungs of male BALB/c nude mice by the intrathoracic injection method. The lung and other organs, including brain, liver, spleen, kidney, muscle, adrenal gland, and lymph node on knee, were removed and stained with H/E to detect the presence of tumor cells. RESULTS: The reliable tumorigenicity time in the PC14PE6 adenocarcinoma cell-inoculated BALB/c nude mouse was 10 days after intrathoracic injection. The average life span of the three groups after inoculation was 14 days in the 2x10(6) cells inoculum group; 25 days in the 1x10(6) cells inoculum group; and 32 days in the 0.5x10(6) cells inoculum group. The PC14PE6 adenocarcinoma cells induced orthotopic lung cancer limited within the thorax. CONCLUSIONS: This orthotopic lung cancer model is an efficient cancer model with easy inoculation methods, rapid and high tumorigenicity, and simple monitoring methods for metastasis.
