Distribution and characterization of IL-10-secreting cells in lymphoid tissues of PCV2-infected pigs.
10.4142/jvs.2010.11.3.177
- Author:
Alan R DOSTER
1
;
Sakthivel SUBRAMANIAM
;
Ji Young YHEE
;
Byung Joon KWON
;
Chi Ho YU
;
So Young KWON
;
Fernando A OSORIO
;
Jung Hyang SUR
Author Information
1. School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska 68583, USA.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
B-cells;
immunohistochemistry;
interlukin-10;
porcine circovirus type 2;
T-cells
- MeSH:
Animals;
Circoviridae Infections/immunology/pathology/*veterinary;
Circovirus/*immunology;
Gene Expression Regulation/*immunology;
Immunohistochemistry/veterinary;
Interleukin-10/immunology/*secretion;
Lymphoid Tissue/immunology/*pathology/secretion;
Porcine Postweaning Multisystemic Wasting Syndrome/*immunology/pathology;
Republic of Korea;
Swine;
T-Lymphocytes/immunology
- From:Journal of Veterinary Science
2010;11(3):177-183
- CountryRepublic of Korea
- Language:English
-
Abstract:
Distribution and characterization of interlukin-10 (IL-10)-secreting cells in lymphoid tissues of pigs naturally infected with porcine circovirus type 2 (PCV2) were evaluated in accordance with PCV2 antigen detection. After screening a total of 56 pigs showing the symptoms of postweaning multisystemic wasting syndrome (PMWS), 15 pigs were PCV2 positive and 5 pigs, which showed stronger positive signals over multiples tissues were further investigated. This study showed that in PCV2-infected lymphoid tissues, particularly mandibular lymph node, spleen and tonsil, IL-10 expression was mainly localized in T-cell rich areas but rarely in B cell rich areas. IL-10 was highly expressed in bystander cells but rarely in PCV2-infected cells. Elevated IL-10 expression was predominantly associated with T cells, but rarely with B cells or with macrophages. The results of this study provide evidence for the role of IL-10 in chronic PCV2 infection and its relation to PCV2 antigen in affected tissues. Constantly elevated levels of IL-10 lead to immunosuppression in persistent and chronic viral infections. The increased IL-10 expression observed in PCV2 infection in this study suggests that IL-10-mediated immunosuppression may play an important role in the pathogenesis and maintenance of naturally occurring PCV2 infection.
