Expression of 14-3-3 proteins and Bax in the placenta of preeclampsia.
- Author:
Ji Kwon PARK
1
;
Seon Im KIM
;
Won Young PAIK
Author Information
1. Department of Obstetrics and Gynecology, College of medicine, Gyeongsang National university, JinJu, Korea. wypaik@gnu.ac.kr
- Publication Type:Original Article
- Keywords:
Preeclampsia;
Placenta;
14-3-3 Proteins;
Bax Protein
- MeSH:
14-3-3 Proteins*;
bcl-2-Associated X Protein;
Blotting, Western;
Female;
Humans;
Immunoprecipitation;
Placenta*;
Pre-Eclampsia*;
Pregnant Women
- From:Korean Journal of Obstetrics and Gynecology
2007;50(6):858-864
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: Purpose of our study was to examine the expression level of 14-3-3 proteins and Bcl-2 family and to estimate the interaction between 14-3-3 proteins and Bcl-2 family in normal and preeclamptic placenta. METHODS: Placental tissues were sampled from preeclampsia with caesarean delivery (n=5) and normal pregnant women with caesarean delivery (n=5). Western blot and immunoprecipitation related Western blotting were performed on all placental samples. Unpaired Student t-test was used to determine the statistical significance. RESULTS: Western blot analysis revealed that the expression of Bax and 14-3-3 zeta was significantly greater in the preeclamptic placenta than in the normal placenta. Immunoprecipitation related Western blotting revealed that the interaction between 14-3-3 zeta and Bax was significantly less in the preeclamptic placenta than in the normal placenta. CONCLUSION: Increased expression of Bax and reduced interaction (between) 14-3-3 zeta and Bax in preeclamptic placenta might influence pathogenesis or sequelae of preeclampsia. Further study is needed to identify the trigger that induces dissociation of Bax from 14-3-3 proteins.
