Etiological characteristics and molecular typing of food poisoning incidents by Vibrio parahaemolyticus in Fuqing City, Fujian Province from 2021 to 2023
- Author:
WANG Juan
;
LIU Xiufeng
;
CHEN Fanbing
;
CHEN Weiwei
;
PAN Jieru
- Publication Type:Journal Article
- Keywords:
Vibrio parahaemolyticus;
food-borne disease outbreak;
serotype;
PFGE;
virulence genes;
antibiotic resistance
- From:
China Tropical Medicine
2024;24(6):740-
- CountryChina
- Language:Chinese
-
Abstract:
Abstract: Objective To analyze the etiological characteristics of Vibrio parahaemolyticus (VP) isolated from food-borne outbreaks in Fuqing City between 2021 and 2023, and to explore its distribution characteristics and epidemic types. Methods The serotypes, virulence genes (tlh, tdh, trh), and drug resistance profiles of 23 VP strains isolated from 4 food-borne disease outbreaks were tested by agglutination, multiple real-time PCR, and broth microdilution methods. Pulsed-field gel electrophoresis (PFGE) was used for molecular typing to analyze homology. Results From 2021 to 2023, four food-borne disease outbreaks occurred in Fuqing City, leading to the isolation and identification of 23 VP strains. Among these, 19 clinical strains were divided into two serotypes: O10:K4 type, comprising 13 strains (68.4%), and O4:KUT type, comprising 6 strains (31.6%); the tdh gene carrying rate was 100.0%, and no trh gene was detected. The serotypes of the four food isolates were O3∶K4, O10:K24, O11∶KUT, and O3∶K37, respectively, all differing from the clinical strains, with two strains coming from the same food item without tdh and trh genes detected. The resistance rate of all isolates to cefazolin was the highest (87.0%), with two strains (8.7%) from different sources showing multidrug resistance. The PFGE genotypes of 19 case isolates were clustered and correlated with their serotypes and drug resistance profiles; strains isolated from the same outbreak event showed high similarity, whereas food strains exhibited polymorphism in PFGE genotypes. Conclusions The predominant serotype causing the four outbreaks was O10:K4, with some strains showing multidrug resistance; all clinical strains carried the tdh gene. The high correlation between strains from the same outbreak event suggests they are homologous. Special attention should be paid to the risk of outbreaks and pandemics caused by the O10:K4 type strain again. Additionally, food safety risk monitoring and assessment should be strengthened to provide an effective basis for epidemic prevention and control and early identification and warning.
- Full text:202506181029498304218.Etiological characteristics and molecular typing of food poisoning incidents by Vibrio parahaemolyticus in Fuqing City, Fujian Province from 2021 to 2023.pdf
