Survey of mosquito species and analysis of genetic structure of important mosquito species populations in Wuyi Mountain Nature Reserve
- Author:
WANG Ming
;
XIE Jingwen
;
DU Yutong
;
WANG Gang
;
YIN Zige
;
CAI Jinghong
;
LIU Qing
;
ZHANG Hengduan
;
ZHAO Tongyan
;
SHI Li
- Publication Type:Journal Article
- Keywords:
Mosquito;
Wuyi Mountain;
CO Ⅰ gene sequence;
CO Ⅱ gene sequence;
species identification;
population genetic structure
- From:
China Tropical Medicine
2024;24(5):499-
- CountryChina
- Language:Chinese
-
Abstract:
bstract: Objective To elucidate the distribution characteristics of mosquito species and the population genetic
diversity of important mosquito species in the Wuyi Mountain Nature Reserve, with the aim of providing a scientific basis for
the prevention and control of mosquito-borne diseases in the region. Methods Within Wuyi Mountain Nature Reserve 2022-
2023, 16 sampling sites were selected, where larvae were collected using the pipette method and adult mosquitoes were
collected using the mosquito trap lamp method. Mosquito species identification was accomplished by integrating morphological
characteristics and molecular identification techniques based on CO Ⅰ and CO Ⅱ gene sequences. Software tools such as
ClustalX, DnaSP v5.0, and Network v4.6.1.0 were utilized to analyze the genetic differentiation (Fst), gene flow (Nm) among
populations of Aedes japonicus, Aedes albopictus and Armigeres subalbatus. Results The mosquito specimens collected from
the Wuyi Mountain Nature Reserve were identified as 30 species belonging to 8 genera within the Culicidae family. Among
them, there were 10 species of Aedes, 13 species of Culex, 1 species each of Armigeres, Orthopodomyia, Uranotaenia,
Tripyeroides, and Anopheles, and 2 species of Toxorhynchites. Analysis of the genetic structure of important mosquito
populations showed that the haplotype diversity index (Hd) of Ae. japonicus was 0.994 7, with a generally moderate degree of
differentiation between populations and a higher degree of genetic differentiation between populations 6 and 12; Aedeslbopictus had a haplotype diversity index of 0.634 0, with significant genetic differentiation between populations 1 and 2
compared to other groups; Armigeres subalbatus had a haplotype diversity index of 0.703 3, with substantial genetic
differentiation and limited gene flow between population 2 and populations 3, 4, 6, and 7. Conclusions The mosquito species
are rich in Wuyi Mountain Nature Reserve. A comprehensive survey of the composition and distribution of mosquito populations
was carried out, and the characteristics of the population genetic structure of the important species of Ae. japonicus, Ae.
Albopictus, and Ar. subalbatus populations were analyzed, providing valuable scientific data reference for local mosquito-borne
ecological research and disease prevention and control.
- Full text:20250617165214572771.Survey of mosquito species and analysis of genetic structure of important mosquito species populations in Wuyi Mountain Nature Reserve.pdf
