Molecular Identification Of Oriental Medicinal Plant Schizonepeta Tenuifoliabunge By Multiplex PCR
- VernacularTitle:Molecular Identification Of Oriental Medicinal Plant Schizonepeta Tenuifoliabunge By Multiplex PCR
- Author:
Baigalmaa J
1
;
Deok-Chun Yang
1
Author Information
1. Mongolian National University Of Medical Sciences
- Publication Type:Journal Article
- Keywords:
Schizonepeta tenuifolia;
agastache rugosa;
elsholtzia ciliate;
multiplex PCR;
single nucleotide polymorphism(SNP);
trn L-F
- From:
Journal of Oriental Medicine
2014;6(2):52-59
- CountryMongolia
- Language:English
-
Abstract:
Schizonepeta tenuifolia (Korean name Hyung-Gae) is an oriental
medicinal plant that is widely used in Korea, China and Japan. S.
tenuifolia (Hyung-Gae) has many pharmacological activities and is
mostly used for many medicinal preparations. The dried aerial part
(spikes and stems) of three oriental medicinal plants S. tenuifolia
(Hyung-Gae), Agastache rugosa(Kwhak-Hyang) and Elsholtzia
ciliata(Hyang-Yoo) belonging to the same family, mint family
Labiaceae, have so similar shape and smell, so it is not easy to
differentiate the three medicinal plants. trnL-F region of chloroplast
DNA of the three medicinal plants were sequenced and used as a
target in multiplex PCR reaction to identify S. tenuifolia. After alignment
of trnL-F sequences of the authenticated plant samples, one single
nucleotide polymorphism specific to S. tenuifoliawas found. Based on
this SNP, new primer was designed that specifically amplify trnL-F
region of S. tenuifolia. The established multiplex-PCR was proven to be
effective in the differentiation of commercial S. tenuifoliamaterials from
A. rugosaand E. ciliata. This rapid and accurate molecular method is
highly promising for use in the food industry.
