Localization Of Collagen Fiber In Human Liver
- Author:
Purevsuren Kh
1
;
Enebish S
1
;
Badamjav S
1
;
Dagdanbazar B
1
;
Amgalanbaatar D
1
Author Information
1. Department of Anatomy, School of Biomedicine, Health Sciences University of Mongolia
- Publication Type:Journal Article
- Keywords:
Collagen fibrillar network of the liver
- From:
Journal of Oriental Medicine
2012;3(2):35-35
- CountryMongolia
- Language:English
-
Abstract:
Background: The collagen fibrillar framework of the liver is
presumed not only to mechanically support the tissue, but also to
form a microenvironment for hepatocytes and cells in the Disse’s
space. It has long been considered that the collagen fibers in the
liver provide the endothelial cells of the hepatic sinusoids with a
scaffold, while their excessive accumulation in the hepatic
parenchyma is associated with the chronicity of liver diseases and
the development of cirrhosis.
Purpose: To define detailed organization of the collagen fibrils of the
liver.
Materials and methods: Collagen fibrils were taken from the livers of
5 male and female cadavers who died not from liver diseases at the
ages from 35 to 55. Light microscopy of hematoxylin-eosin stained
liver tissues confirmed that the materials had no significant
pathological changes. The collagen fibrils of the liver were extracted
and observed according to our cell-maceration/SEM method.
Results: The alkali-water maceration of the liver at room
temperature consistently removed all of the cellular elements with
their basal lamina, and left collagen fibrils in their natural locations
and shapes, thus revealing the continuum of the collagen fibrillar
network under the SEM. The diameter of collagen fibrils was around
57 nm. The collagen fibrillar layer of the liver capsule was about 70-
100 µm in thickness. The capsular layer consisted of collagen
fibrillar bundles (0.2-4µm in diameter) of densely packed parallel
collagen fibrils. Deriving from between such bundles were many
collagen fibrils, they wound independently for some distance and
then again fused with the bundles. There were many bundles of
collagen fibrils that connected the capsular collagen layer to the
collagen fibrillar sheaths for housing the hepatic sinusoids. There
were no collagen fibrillar septa delimiting the hepatic lobules,
instead, collagen fibrillar sheaths for the sinusoids were continuum
throughout the liver. Normal human livers contained 12.0 mg of
collagen fibrils/g wet tissue. Both type I and III collagens have been
demonstrated immunehistochemically in normal liver.
