Analysis of CCL5 Concentration in Tears of Dry Eye Patients.
10.3341/jkos.2011.52.6.658
- Author:
Won CHOI
1
;
Seung Hyun LEE
;
Kyung Chul YOON
Author Information
1. Department of Ophthalmology, Chonnam National University Hospital, Chonnam National University Medical School, Gwangju, Korea. kcyoon@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
CCL5/RANTES;
Dry eye;
Ocular surface;
Sjogren's syndrome;
Tear film
- MeSH:
Chemokine CCL5;
Enzyme-Linked Immunosorbent Assay;
Eye;
Goblet Cells;
Humans;
Sensation;
Sjogren's Syndrome;
Tears
- From:Journal of the Korean Ophthalmological Society
2011;52(6):658-664
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the expression of CCL5/RANTES (regulated upon activation, normal T cell expressed and secreted) in the tears of dry eye patients. METHODS: Forty patients with dry eye (15 Sjogren's and 25 non-Sjogren's syndrome patients) and ten control subjects were recruited for the present study. The concentration of RANTES in tears was measured using an enzyme-linked immunosorbent assay. The correlations between RANTES level, tear film and ocular surface parameters, including tear film break-up time, basal tear secretion, tear clearance rate, corneal sensation, keratoepitheliopathy, and conjunctival goblet cell density, were analyzed in patients with dry eye syndrome. RESULTS: The concentrations of RANTES were 435.46 +/- 104.45 pg/ml in Sjogren's syndrome patients, 257.42 +/- 46.72 pg/ml in non-Sjogren's syndrome patients, and 97.53 +/- 29.15 pg/ml in the control patients (p < 0.01). The levels correlated significantly with basal tear secretion, tear clearance rate, keratoepitheliopathy, and goblet cell density (p < 0.05). CONCLUSIONS: CCL5/RANTES level increases in the tears of dry eye patients and correlates with various tear film and ocular surface parameters.
