Effects of Mitomycin C on Scleral Collagen Fibrils According to Atomic Force Microscopy.
10.3341/jkos.2011.52.6.671
- Author:
Hui Jae LEE
1
;
Samjin CHOI
;
Youjin CHEONG
;
Gyeong Bok JUNG
;
Kyung Hyun JIN
;
Hun Kuk PARK
;
Seung Jun LEE
Author Information
1. Department of Ophthalmology, School of Medicine, Kangwon National University, Chuncheon, Korea. opticus@kangwon.ac.kr
- Publication Type:Original Article
- Keywords:
Atomic force microscopy;
Collagen fibrils;
Diameter;
D-banding;
Human sclera;
Mitomycin C
- MeSH:
Collagen;
Humans;
Microscopy, Atomic Force;
Mitomycin;
Sclera
- From:Journal of the Korean Ophthalmological Society
2011;52(6):671-678
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the effects of mitomycin C on the scleral collagen surfaces using atomic force microscopy (AFM). METHODS: Two non-contact mode AFM machines were used to observe changes in the morphological characteristics of human scleral surfaces before and after one, three, and five minutes of 0.02% mitomycin C application. Based on AFM topography and deflection images of the collagen fibril, the morphological characteristics of scleral fibrils including the fibril diameter and D-period were measured using the line profile. RESULTS: The sclera collagen fibril treated with 0.02% mitomycin C for one minute did not show any significant increases in mean fibril diameter (155.04 +/- 17.46 nm) or mean D-periodicity (70.02 +/- 3.33 nm), compared to those of the control group. However, the scleral collagen fibrils treated with 0.02% mitomycin C for three and five minutes showed significant increases in mean fibril diameter (182.33 +/- 16.33 nm, 199.20 +/- 12.40 nm, respectively) and mean D-periodicity (70.27 +/- 13.66 nm, 72.75 +/- 19.32 nm, respectively), compared to those of the control group. CONCLUSIONS: The present study examined the structural changes in the scleral collagen fibrils before and after mitomycin C application according to atomic force microscopy. The results indirectly suggest that three or more minutes of 0.02% mitomycin C application affects the morphology of scleral collagen.
