Differentially expressed genes of Acanthamoeba castellanii during encystation.
10.3347/kjp.2007.45.4.283
- Author:
Eun Kyung MOON
;
Dong Il CHUNG
;
Yeon Chul HONG
;
Hyun Hee KONG
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
Acanthamoeba;
differentially expressed gene;
encystation
- MeSH:
Acanthamoeba castellanii/*genetics/*growth & development;
Amino Acid Sequence;
Animals;
*Gene Expression Profiling;
Gene Expression Regulation;
*Life Cycle Stages;
Molecular Sequence Data;
Protozoan Proteins/*genetics;
Reverse Transcriptase Polymerase Chain Reaction;
Sequence Alignment;
Sequence Homology, Amino Acid;
Up-Regulation
- From:The Korean Journal of Parasitology
2007;45(4):283-285
- CountryRepublic of Korea
- Language:English
-
Abstract:
To examine the expressed gene profile during encystation of Acanthamoeba castellanii Castellani, we used differentially expressed gene (DGE) screening by RT-PCR with 20 sets of random primers. From this analysis, we found that approximately 16 genes showed upregulation during encystation. We chose 6 genes, which had relatively higher expression levels, for further investigation. Based on homology search in database, DEG2 showed 55% of similarity with xylose isomerase, DEG9 showed 37% of similarity with Na P-type ATPase, and DEG14 showed 77% of similarity with subtilisin-like serine proteinase. DEG3 and DEG26 were identified as hypothetical proteins and DEG25 exhibited no significant similarity to any known protein. Encystation of Acanthamoeba has been suggested to be a process to resist adverse environmental or nutritional conditions. Further characterization studies of these genes may provide us with more information on the encystation mechanism of Acanthamoeba.
