Loss of infectivity of Neospora caninum oocysts maintained for a prolonged time.
10.3347/kjp.2007.45.4.295
- Author:
Rosangela Soares UZEDA
;
Kattyanne Souza COSTA
;
Sara Lima SANTOS
;
Alexandre Moraes PINHEIRO
;
Maria Angela Ornelas ALMEIDA
;
Milton M MCALLISTER
;
Luis Fernando Pita GONDIM
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
Neospora caninum;
oocysts;
Mongolian gerbils;
Meriones unguiculatus;
infectivity
- MeSH:
Acids;
Animals;
Brain/parasitology/pathology;
Cattle/parasitology;
Coccidiosis/parasitology/pathology/*veterinary;
Feces/parasitology;
Female;
Gerbillinae/*parasitology;
Neospora/genetics/growth & development/*pathogenicity;
Oocysts/*growth & development;
Polymerase Chain Reaction/methods;
Refrigeration;
Virulence
- From:The Korean Journal of Parasitology
2007;45(4):295-299
- CountryRepublic of Korea
- Language:English
-
Abstract:
The purpose of this study was to investigate whether sporulated Neospora caninum oocysts, which had been stored for 46 mo in a 2% sulfuric acid solution at 4 degrees C, remain morphologically viable and infective to gerbils (Meriones unguiculatus). Six gerbils were orally inoculated with doses of 400 or 1,200 oocysts. Two mo after inoculation, the animals did not show any clinical signs, had no histological lesions, and were seronegative for N. caninum at 1: 50 in an immunofluorescent antibody test. PCR using the brain from each gerbil did not reveal N. caninum specific DNA. We conclude that oocysts preserved for 46 mo are not infective, despite being morphologically intact.
