Expression of ras Oncogene in Tumor Cells of Epidermis.
- Author:
Sung Kyun KIM
;
Kyu Chul CHOI
;
Sun Wook HWANG
- Publication Type:Original Article
- Keywords:
ras oncogene;
Epidermal tumors
- MeSH:
Animals;
Bowen's Disease;
Carcinogenesis;
Carcinoma, Squamous Cell;
Cytoplasm;
DNA;
Epidermis*;
Genes, ras*;
Humans;
Keratoacanthoma;
Keratosis, Actinic;
Keratosis, Seborrheic;
Oncogenes;
Biomarkers, Tumor
- From:Korean Journal of Dermatology
1988;26(2):153-159
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
DNA isolated from human tumor cells can induce malignant transfarmation of tissue culture cells. The DNA is then called an oncogene. Its protein produets have been detected in animal and human tumors and are considered to play a significant role in carcinogenesis. In order to evaluate whether the oncogenes are involved in development of tumors of epidermis and whether they could be used as tumor markers, immunoperoxidase staining was performed for the ras product in sections of squamous cell carcinoma, Bowen's disease, actinic keratosis, keratoacanthoma and seborrheic keratosis. Three cases of sgamous cell carcinoma showed 10~20 positive cells per high power field(HPF). Three cases of Bowen's disease revealed 1-9 positive cells per HPF, whereas the actinic keratosis 1~9 or no positive cells per 10 HPF in all three cases. The keratoacanthoma and seborrheic keratosis showed 1~9 or no positive cells in all observed cases. The positive staining was observed in the cytoplasm. The increasing positivity in parallel with the increase of malignant potential strongly suggests that the ras oncogene is closely related to development of epidermal malignancy and also point out the possibility of ras as a cancer marker.
