Clinical Approaches for Understanding the Expression Levels of Pattern Recognition Receptors in Otitis Media with Effusion.
- Author:
So Yoon LEE
1
;
Eun Woong RYU
;
Jong Bin KIM
;
Seung Geun YEO
Author Information
1. Department of Otorhinolaryngology, Kyung Hee University School of Medicine, Seoul, Korea. yeo2park@yahoo.co.kr
- Publication Type:Original Article
- Keywords:
Otitis media with effusion;
Pattern recognition receptors;
Innate immunity
- MeSH:
Bacteria;
Bacterial Infections;
Ear, Middle;
Exudates and Transudates;
Humans;
Immune System;
Immunity, Innate;
Otitis;
Otitis Media;
Otitis Media with Effusion;
Polymerase Chain Reaction;
Porcine Reproductive and Respiratory Syndrome;
Proteins;
Receptors, Pattern Recognition;
RNA, Messenger;
Toll-Like Receptors;
Ventilation
- From:Clinical and Experimental Otorhinolaryngology
2011;4(4):163-167
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVES: Bacterial infections in the normally sterile environment of the middle ear cavity usually trigger host immune response, whereby the innate immune system plays a dominant role as the host's first line of defense. We evaluated the expression levels of Toll-like receptors (TLRs) -2, -4, -5, -9, and nucleotide-binding oligomerization domain-containing proteins (NODs) -1 and -2, all of which are related to bacterial infection in pediatric patients with otitis media with effusion (OME). METHODS: The study sample consisted of 46 pediatric patients with OME, all of whom had ventilation tubes inserted. The expression levels of TLR-2, -4, -5, -9, NOD-1 and -2 mRNA in middle ear effusion were assessed by polymerase chain reaction (PCR). Difference of pattern recognition receptors (PRRs) expression level by presence of bacteria, ventilation tube insertion rate, and effusion fluid character was assessed. RESULTS: All effusion fluid samples collected from patients with OME showed expression of TLR-2, -4, -5, -9, NOD-1, and -2 mRNA by PCR. However, we found no differences among expression levels of PRRs in relation to characteristics of exudates, presence of bacteria, or frequencies of ventilation tube insertion (P>0.05). CONCLUSION: Our findings suggest that exudates of OME patients show PRR expressions that are related to the innate immune response regardless of the characteristics of effusion fluid, presence of bacteria in exudates, or frequency of ventilation tube insertion.
