Isolation and identification of aerobic and anaerobic bacteria from the feces of wild Korean water deer (Hydropotes inermis argyropus)
- Author:
Md Ashraful ISLAM
1
;
Sungryong KIM
;
Md Sodrul ISLAM
;
Obaidul ISLAM
;
Seunghyeon PARK
;
Itainara TAILI
;
Dong-Hyuk JEONG
;
Ki-Jeong NA
Author Information
- Publication Type:Research Report
- From:Journal of Veterinary Science 2024;25(6):e78-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Objective:This study aims to isolate and identify aerobic and anaerobic bacterial species in the feces of wild KWD.
Methods:Fecal samples were collected from 55 wild KWD of varying age and sex.Aerobic bacteria were cultured at 37°C for 24–48 h under standard conditions, whereas anaerobic bacteria were cultured at 37°C for 48–72 h in an anaerobic environment. Bacterial identification was conducted using DNA extraction and polymerase chain reaction amplification targeting the 16S rRNA gene.
Results:The predominant aerobic bacteria identified belonged to the Firmicutes (58.18%) and Proteobacteria (41.82%) phyla, with Escherichia coli (31.82%) and Bacillus cereus (31.82%) being the most common species. Among anaerobic bacteria, most belonged to the Firmicutes (71.03%), Proteobacteria (27.10%), and Fusobacteriota (1.87%) phyla, with Paraclostridium bifermentans (28.97%) and E. coli (22.43%) being the most prevalent species.Other frequently identified anaerobic species were Fusobacterium varium, Lactococcus garvieae, Terrisporobacter glycolicus, Enterococcus faecalis, and Clostridium sporogenes.
Conclusions:and Relevance: Our findings indicate a diverse microbial community in the feces of water deer, offering valuable insights into their gut microbiota and its potential implications for health and ecology.
