Molecular cloning and characterization of porcine ribosomal protein L21.
10.4142/jvs.2017.18.4.531
- Author:
Wu Sheng SUN
1
;
Ju Lan CHUN
;
Dong Hwan KIM
;
Jin Seop AHN
;
Min Kyu KIM
;
In Sul HWANG
;
Dae Jin KWON
;
Seongsoo HWANG
;
Jeong Woong LEE
Author Information
1. Biotherapeutics Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea. jwlee@kribb.re.kr
- Publication Type:Original Article
- Keywords:
Sus scrofa;
conservation;
gene expression;
molecular cloning;
ribosomal protein L21
- MeSH:
Amino Acids;
Cell Cycle Checkpoints;
Cell Proliferation;
Chromosomes, Human, Pair 11;
Clone Cells;
Cloning, Molecular*;
Female;
Gene Expression;
Genetic Diseases, Inborn;
Heart;
Intestine, Small;
Kidney;
Liver;
Lung;
Open Reading Frames;
Ovary;
Ribosomal Proteins*;
Ribosomes;
Skin;
Sus scrofa
- From:Journal of Veterinary Science
2017;18(4):531-540
- CountryRepublic of Korea
- Language:English
-
Abstract:
Ribosomal protein L21 (RPL21) is a structural component of the 60S subunit of the eukaryotic ribosome. This protein has an important role in protein synthesis and the occurrence of hereditary diseases. Pig is a common laboratory model, however, to the best of our knowledge, its RPL21 gene has not been cloned to date. In this study, we cloned and identified the full-length sequence of the pig RPL21 gene for the first time. In addition, we examined its expression pattern and function by using overexpression or knockdown approaches. As a result, we obtained a 604 bp segment that contains a 483 bp open reading frame encoding 160 amino acids. The pig RPL21 gene is located in the “+” strand of chromosome 11, which spans 2167 bp from 4199792 to 4201958. Pig RPL21 protein has nine strands and two helices in its secondary structure. Pig RPL21 is predominantly expressed in ovary and lung, at lower levels in kidney, small intestine, and skin, and at the lowest levels in heart and liver. Furthermore, RPL21 expression is closely connected with cell proliferation and cell cycle arrest. The results are intended to provide useful information for the further study of pig RPL21.
