Surveyor assay to diagnose persistent Müllerian duct syndrome in Miniature Schnauzers.
10.4142/jvs.2017.18.4.547
- Author:
Young June KIM
1
;
Hyuk Jin KWON
;
Hyuk Soo BYUN
;
Donguk YEOM
;
Jea Hong CHOI
;
Joong Hyun KIM
;
Hosup SHIM
Author Information
1. Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 31116, Korea. shim@dku.edu
- Publication Type:Brief Communication
- Keywords:
Miniature Schnauzer;
persistent Müllerian duct syndrome;
surveyor nuclease assay
- MeSH:
Animals;
Diagnosis;
Digestion;
Disorders of Sex Development;
DNA;
Dogs;
Humans;
Male;
Methods;
Pathology, Molecular
- From:Journal of Veterinary Science
2017;18(4):547-549
- CountryRepublic of Korea
- Language:English
-
Abstract:
Persistent Müllerian duct syndrome (PMDS) is a pseudohermaphroditism in males characterized by the presence of Müllerian duct derivatives. As PMDS dogs often lack clinical symptoms, a molecular diagnosis is essential to identify the syndrome in these animals. In this study, a new molecular method using DNA mismatch-specific Surveyor nuclease was developed. The Surveyor nuclease assay identified the AMHR2 mutation that produced PMDS in a Miniature Schnauzer as accurately as that obtained by using the conventional method based on restriction digestion. As an alternative to the current molecular diagnostic method, the new method may result in increased accuracy when detecting PMDS.
