Effects of Tributyltin Acetate on the Testicular Expression of Steroidogenic Enzyme Genes in Immature Mouse Testes.
- Author:
Ho Seung KIM
1
;
Suel Ki KIM
;
Juri HAN
;
Chang Joo LEE
;
Jae Seong LEE
;
Yong Dal YOON
Author Information
1. Department of Life Science, College of Natural Sciences, Hanyang University, Korea. ydyoon@hanyang.ac.kr
- Publication Type:Original Article
- Keywords:
Tributyltin acetate;
Testosterone;
Testis
- MeSH:
Animals;
Aromatase;
Cytochrome P-450 Enzyme System;
Estradiol;
Gonads;
Humans;
Male;
Mice*;
Oxidoreductases;
Radioimmunoassay;
Reproduction;
RNA, Messenger;
Testis*;
Testosterone
- From:Korean Journal of Andrology
2005;23(2):80-87
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The present study was performed to evaluate the effects of tributyltin acetate(TBTA) on mouse testes. The effects of TBTA on mammalian reproduction are not well known. MATERIALS AND METHODS: Three-week-old male mice(ICR strain) were orally administered TBTA at doses of 0 (control vehicle, CV), 25(T25), 50(T50), and 100 mg/kg(T100). Serum and intratesticular concentrations of testosterone and estradiol were determined by conventional radioimmunoassays. RT-PCR analysis was also performed. RESULTS: Transcriptional activity of 3-hydroxysteroid dehydrogenase (3beta-HSD), 17-hydroxysteroid dehydrogenase(17 beta-HSD) and cytochrome P450 17alpha-hydroxylase/C17,20 lyase(P450 (17 alpha)) were decreased by treatment. whereas mRNA levels of P450 aromatase were unaffected. In addition, TBTA significantly decreased serum testosterone levels in T100, while estradiol levels were not affected significantly. CONCLUSIONS: Administration of TBTA decreases testosterone level in testes, and this effect might be due to the alteration of mRNA levels of steroidogenic enzymes. Taken together, these findings suggest that TBTA, impairs testicular functions in a dose-dependent manner. The present results can be used as basic data in the study of TBTA action on gonads.
