Padina arborescens extract protects high glucose-induced apoptosis in pancreatic beta cells by reducing oxidative stress.
- Author:
Mi Hwa PARK
1
;
Ji Sook HAN
Author Information
- Publication Type:Original Article
- Keywords: Padina arborescens extract; high glucose; apoptosis; oxidative stress; INS-1 pancreatic beta cell
- MeSH: Apoptosis*; Blotting, Western; Caspase 3; Cell Death; Cell Survival; Glucose; Insulin; Insulin-Secreting Cells*; Lipid Peroxidation; Oxidative Stress*
- From:Nutrition Research and Practice 2014;8(5):494-500
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND/OBJECTIVES: This study investigated whether Padina arborescens extract (PAE) protects INS-1 pancreatic beta cells against glucotoxicity-induced apoptosis. MATERIALS/METHODS: Assays, including cell viability, lipid peroxidation, generation of intracellular ROS, NO production, antioxidant enzyme activity and insulin secretion, were conducted. The expressions of Bax, Bcl-2, and caspase-3 proteins in INS-1 cells were evaluated by western blot analysis, and apoptosis/necrosis induced by high glucose was determined by analysis of FITC-Annexin V/PI staining. RESULTS: Treatment with high concentrations of glucose induced INS-1 cell death, but PAE at concentrations of 25, 50 or 100 microg/ml significantly increased cell viability. The treatment with PAE dose dependently reduced the lipid peroxidation and increased the activities of antioxidant enzymes reduced by 30 mM glucose, while intracellular ROS levels increased under conditions of 30 mM glucose. PAE treatment improved the secretory responsiveness following stimulation with glucose. The results also demonstrated that glucotoxicity-induced apoptosis is associated with modulation of the Bax/Bcl-2 ratio. When INS-1 cells were stained with Annexin V/PI, we found that PAE reduced apoptosis by glucotoxicity. CONCLUSIONS: In conclusion, the present study indicates that PAE protects against high glucose-induced apoptosis in pancreatic beta cells by reducing oxidative stress.
