Comparison of FcRgamma-Deficient and CD57+ Natural Killer Cells Between Cord Blood and Adult Blood in the Cytomegalovirus-Endemic Korean Population.
10.3343/alm.2015.35.4.423
- Author:
Hee Jo BAEK
1
;
Da Woon KIM
;
Minh Trang Thi PHAN
;
Ju Sun KIM
;
Ji Hoon YANG
;
Jeong Il CHOI
;
Je Jung LEE
;
Myung Geun SHIN
;
Dong Wook RYANG
;
Sang Ki KIM
;
Seung Hwan LEE
;
Hoon KOOK
;
Duck CHO
Author Information
1. Department of Pediatrics, Chonnam National University Hwasun Hospital, Chonnam National University Medical School, Gwangju, Korea. hoonkook@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
Adult;
Blood;
Cord;
Cytomegalovirus;
Infection;
FcRgamma;
Natural killer cell
- MeSH:
Adult*;
Cytomegalovirus;
Fetal Blood*;
Flow Cytometry;
Humans;
Killer Cells, Natural*
- From:Annals of Laboratory Medicine
2015;35(4):423-428
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: FcRgamma-deficient natural killer (NK) cells (g-NK cells) have been associated with cytomegalovirus (CMV) infection. However, the frequency of g-NK cells in a CMV-endemic area (i.e., Korea) has not yet been studied. We examined the frequency of g-NK cells and expression of CD57 on NK cells in cord blood (CB) and adult blood (AB). METHODS: Of the 24 AB samples collected, 95.8% (23/24) were CMV IgG+/IgM-, while 100% of the 13 healthy CB samples were CMV IgG+/IgM-. We performed whole-blood flow cytometry assays to analyze intracellular FcRgamma and CD3zeta expression of CD3-/CD56dim NK cells from 13 CB and 24 AB samples, and surface CD57 expression on CD3-/CD56dim/CD16+ NK cells from 13 CB and 19 AB samples. RESULTS: All CMV seropositive AB samples contained g-NK cells (23/23), and the median proportion of g-NK cells in the CD3-/CD56dim NK cell pool was 35.0% (range: 11-77%). CD57+ NK cells in the CD3-/CD56dim/CD16+ NK cell population were detected in all 19 AB samples tested, but not in any CB samples. CONCLUSIONS: Our data suggest that g-NK cells and CD57+ NK cells are present at a very high frequency in CMV-seropositive AB, but rare in CMV-naive CB.
