Direct Identification of Staphylococcus aureus and Determination of Methicillin Susceptibility From Positive Blood-Culture Bottles in a Bact/ALERT System Using Binax Now S. aureus and PBP2a Tests.
10.3343/alm.2015.35.4.454
- Author:
Sandrine HERAUD
1
;
Anne Marie FREYDIERE
;
Anne DOLEANS-JORDHEIM
;
Michele BES
;
Anne TRISTAN
;
Francois VANDENESCH
;
Frederic LAURENT
;
Olivier DAUWALDER
Author Information
1. Hospices Civils de Lyon, Laboratoire de Bacteriologie, Centre de Biologie et de Pathologie Est, Bron, France. olivier.dauwalder@chu-lyon.fr
- Publication Type:Brief Communication
- Keywords:
Staphylococcus aureus;
Methicillin resistance;
Blood culture;
Accuracy;
Immunochromatographic test
- MeSH:
Bacteremia;
Diagnosis;
Gram-Positive Cocci;
Methicillin Resistance;
Methicillin*;
Methicillin-Resistant Staphylococcus aureus;
Mortality;
Staphylococcus aureus*
- From:Annals of Laboratory Medicine
2015;35(4):454-457
- CountryRepublic of Korea
- Language:English
-
Abstract:
Staphylococcus aureus bacteremia is associated with high mortality and morbidity, requiring prompt and appropriate antimicrobial treatment. Therefore, it is important to detect methicillin-resistant S. aureus (MRSA) rapidly from blood cultures. Two immunochromatographic tests, BinaxNow S. aureus and BinaxNow PBP2a, were directly applied to 79 Bact/Alert bottles that were positive for Gram positive cocci in cluster aggregations. Sensitivity and specificity for the identification of S. aureus and determination of methicillin resistance were 94% and 87%, and 100% and 100%, respectively, with less than 30 min of performance time. These tests are efficient and rapid; these tests are valuable alternatives to more sophisticated and expensive methods used in the diagnosis of MRSA bacteremia.
