Evaluation of a Rapid Test for Detection of Chlamydia Trachomatis Infection in Female Commercial Sex Workers.
10.4111/kju.2006.47.9.978
- Author:
Gilho LEE
1
;
Jung Hyun SHIM
;
Youngmin BYUN
;
Haekung KIM
Author Information
1. Department of Urology, Dankook University College of Medicine, Cheonan, Korea. prostatitis@hanmail.net
- Publication Type:Original Article
- Keywords:
Chlamydia trachomatis;
Point-of-care systems;
Sex;
Workers
- MeSH:
Chlamydia trachomatis*;
Chlamydia*;
DNA;
Female*;
Genes, vif;
Humans;
Mass Screening;
Plasmids;
Point-of-Care Systems;
Polymerase Chain Reaction;
Prevalence;
Sensitivity and Specificity;
Sex Workers*
- From:Korean Journal of Urology
2006;47(9):978-981
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Purpose: The importance of laboratory screening tests for female commercial sex workers (FCSWs) has been well documented to reduce the prevalence of chlamydial complications. A rapid test has been one of the standard chlamydial tests performed in Korean health centers. Although the process of the rapid test is simple, the sensitivity is inconsistent. Therefore, we evaluated the efficacy of QuickVue chlamydial detection kits, which is one of the rapid tests, by comparing this assay to an in-house polymerase chain reaction (PCR) method. Materials and Methods: A total of 410 endo-cervical samples were consecutively collected in one health center. A rapid test was performed by using a QuickVue kit. Genomic DNA was extracted from cotton swabs. The cryptic plasmid of C. trachomatis from the genomic DNA was amplified by the PCR method. Results: The overall sensitivity, specificity, positive predictive value and negative predictive value of the rapid test were 21%, 99%, 89% and 83%, respectively, based on the PCR results. Study of the serial dilutions of reference inclusion forming units (IFU) showed that the rapid test only detected chlamydial infections that had high counts of IFUs. Conclusions: The rapid test is not good enough to detect chlamydial infection in FCSWs. Instead, a gene amplification test should be used for detecting chlamydial infections in FCSWs.
