Propofol has delayed myocardial protective effects after a regional ischemia/reperfusion injury in an in vivo rat heart model.
10.4097/kjae.2010.58.4.378
- Author:
Il Woo SHIN
1
;
In Seok JANG
;
Seung Hwa LEE
;
Ji Seok BAIK
;
Kyeong Eon PARK
;
Ju Tae SOHN
;
Heon Keun LEE
;
Young Kyun CHUNG
Author Information
1. Department of Anesthesiology and Pain Medicine, Gyeongsang National University College of Medicine, Jinju, Korea. ykchung@nongae.gsnu.ac.kr
- Publication Type:Original Article
- Keywords:
Ischemia/reperfusion injury;
Myocardium;
Propofol
- MeSH:
Animals;
Coronary Vessels;
Emulsions;
Enzyme-Linked Immunosorbent Assay;
Heart;
Heart Ventricles;
Hemodynamics;
Myocardium;
Phospholipids;
Propofol;
Rats;
Reperfusion;
Salicylamides;
Soybean Oil;
Tetrazolium Salts;
Troponin I
- From:Korean Journal of Anesthesiology
2010;58(4):378-382
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: It is well known that propofol protects myocardium against myocardial ischemia/reperfusion injury in the rat heart model. The aim of this study was to investigate whether propofol provides a protective effect against a regional myocardial ischemia/reperfusion injury in an in vivo rat heart model after 48 h of reperfusion. METHODS: Rats were subjected to 25 min of left coronary artery occlusion followed by 48 h of reperfusion. The sham group received profopol without ischemic injury. The control group received normal saline with ischemia/reperfusion injury. The propofol group received profopol with ischemia/reperfusion injury. The intralipid group received intralipid with ischemia/reperfusion injury. A microcatheter was advanced into the left ventricle and the hemodynamic function was evaluated. The infarct size was determined by triphenyltetrazolium staining. The serum level of cardiac troponin-I (cTn-I) was determined by ELISA (enzyme-linked immunosorbent assay). RESULTS: Propofol demonstrated protective effects on hemodynamic function and infarct size reduction. In the propofol group, the +dP/dt(max) (P = 0.002) was significantly improved compared to the control group. The infarct size was 49.8% of the area at risk in the control group, and was reduced markedly by administration of propofol to 32.6% in the propofol group (P = 0.014). The ischemia/reperfusion-induced serum level of cTn-I was reduced by propofol infusion during the peri-ischemic period (P = 0.0001). CONCLUSIONS: Propofol, which infused at clinically relevant concentration during the peri-ischemic period, has delayed myocardial protective effect after regional myocardial ischemia/reperfusion injury in an in vivo rat heart model after 48 h of reperfusion.
