The effective concentration and exposure time of mitomycin-C for the inhibition of lens epithelial cell proliferation in rabbit eyes.
10.3349/ymj.2000.41.2.185
- Author:
Yong Sung YOU
1
;
Seong Jung LIM
;
Hak Sung CHUNG
;
Young Gee LEE
;
Chan Yun KIM
;
Young Jae HONG
Author Information
1. The Institute of Vision Research and Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea. yjhhong0815@yonsei.yumc.ac.kr
- Publication Type:Original Article
- Keywords:
e-crystalline mRNA;
lens epithelial cell;
mitomycin-C;
posterior capsular opacity
- MeSH:
Animal;
Antibiotics, Antineoplastic/pharmacology*;
Cell Division/drug effects;
Cells, Cultured;
DNA/biosynthesis;
Dose-Response Relationship, Drug;
Epithelial Cells/drug effects;
Epithelial Cells/cytology;
Lens, Crystalline/drug effects*;
Lens, Crystalline/cytology;
Mitomycin/pharmacology*;
Rabbits;
Time Factors
- From:Yonsei Medical Journal
2000;41(2):185-189
- CountryRepublic of Korea
- Language:English
-
Abstract:
The proliferation of residual lens epithelial cells following cataract surgery is assumed to be a major cause of posterior capsular opacification. To assess the efficacy of mitomycin-C in preventing posterior capsular opacification, we determined the effective concentration and exposure time of mitomycin-C in inhibiting rabbit lens epithelial cell proliferation. The fourth-passaged rabbit lens epithelial cells were maintained for one day and then exposed to mitomycin-C for 1, 2, 3, and 5 minutes, respectively. There were 9 different plating concentrations of mitomycin-C with two-fold serial dilution. The maintenance of the phenotypic properties of lens epithelial cells was confirmed by continuous transcription of lambda-crystalline mRNA determined by reverse transcription-polymerase chain reaction and the polymorphism of the restriction fragment. Cell proliferation was assayed with 3H-thymidine incorporation into DNA. The fourth-passaged cells maintained the expression of lambda-crystalline mRNA, suggesting that they are phenotypically authentic lens epithelial cells. The effective concentrations and exposure time of mitomycin-C were 0.1 mg/ml for 1 minute and 2 minutes, and 0.025 mg/ml for 2 minutes. By these results, we postulated that mitomycin-C at relatively short incubation times could be clinically used for prevention of posterior capsular opacification after cataract surgery.
