The Effect of Ring-type NF-kappa B(NF-kB) Decoy Oligodeoxynucleotide on the Kidney for an Experimental Unilateral Ureteral Obstruction in Mice.
10.4111/kju.2007.48.8.815
- Author:
Young Gun KWON
1
;
Young Guk LEE
;
Il KANG
;
Eun Suk LEE
;
Kwan Kyu PARK
;
Yoon Seop KEUM
;
Kyung Hyun KIM
;
Jae Shin PARK
Author Information
1. Department of Urology, Daegu Catholic University College of Medicine, Daegu, Korea. jspark@cu.ac.kr
- Publication Type:Original Article
- Keywords:
NF-kappa B;
Transcription factors;
Ureteral obstruction
- MeSH:
Animals;
Cell Adhesion;
Cytokines;
Fibronectins;
Glomerulonephritis;
Humans;
Kidney*;
Male;
Mice*;
Monocytes;
NF-kappa B;
Transcription Factors;
Tumor Necrosis Factor-alpha;
Urea;
Ureter*;
Ureteral Obstruction*
- From:Korean Journal of Urology
2007;48(8):815-825
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The transcription factor, NF-kB, is important in the coordinated expressions of various pro-inflammatory and adhesion molecules. Our hypothesis is that inhibiting the action of NF-kB using a synthetic decoy oligodeoxynucleotide(ODN) can block the underlying inflammatory response in glomerulonephritis. MATERIALS AND METHODS: Forty two male C57BL6 mice, weighting 25g, were divided into four groups; in group 1, 2 mice were used as normal controls; in group 2, a unilateral ureteral obstruction(UUO) was induced in 12 mice; in group 3, 20 UUO mice were treated using ring-type NK-kB decoy ODN; and in group 4, 8 UUO mice were treated using scramble type NF-kB decoy ODN. The mice were killed 1, 3, 5 and 7 days after the NF-kB decoy ODN injections, and the blood urea nitrogen(BUN), and expressions of tumor necrosis factor-alpha (TNF-alpha), interleukin-beta(IL-beta), fibronectin, vascular cell adhesion molecule(VCAM) and monocyte chemotactic protein-1(MCP-1), as well as the histopathological findings, analyzed in each group. RESULTS: The serum levels of BUN, TNF-alpha, IL-beta, fibronectin, VCAM and MCP-1 were increased in group 2, but decreased in group 3. The histopathological findings of the kidneys in group 3 were most similar to those found in the control(group 1). CONCLUSIONS: NF-kB decoy ODN treatment substantially inhibited the disease, with reductions in the histological damage and the renal expressions of inflammatory cytokines.
