Neuroprotective mechanism of corydaline in glutamate-induced neurotoxicity in HT22 cells
10.11620/IJOB.2024.49.1.10
- Author:
Baskar SELVARAJ
1
;
Dae Won KIM
;
Ki-Yeon YOO
;
Keunwan PARK
;
Thi Thu Thuy TRAN
;
Jae Wook LEE
;
Heesu LEE
Author Information
1. Natural Product Research Center, Institute of Natural Product, Korea Institute of Science and Technology, Gangneung 25451, Republic of Korea
- Publication Type:Original Article
- From:International Journal of Oral Biology
2024;49(1):10-17
- CountryRepublic of Korea
- Language:English
-
Abstract:
Glutamate-mediated oxidative stress causes neuronal cell death by increasing intracellular Ca2+ uptake, reactive oxidative species (ROS) generation, mitogen-activated protein kinase (MAPK) activation, and translocation of apoptosis-inducing factor (AIF) to the nucleus. In the current study, we demonstrated that corydaline exerts potent neuroprotective effects against glutamate-induced neurotoxicity. Treatment with 5 mmol/L glutamate increased cellular Ca2+ influx, ROS generation, MAPK activation, and AIF translocation. In contrast, corydaline treatment decreased cellular Ca2+ influx and ROS generation. Western blot analysis revealed that glutamate-mediated MAPK activation was attenuated by corydaline treatment. We further demonstrated that corydaline treatment inhibited the glutamate-mediated translocation of AIF to the nucleus. We propose that corydaline is a promising lead structure for the development of safe and effective neuroprotectants.
