Calcium Uptake and Release through Sarcoplasmic Reticulum in the Inferior Oblique Muscles of Patients with Inferior Oblique Overaction.
10.3349/ymj.2006.47.2.207
- Author:
Hee Seon KIM
1
;
Yoon Hee CHANG
;
Do Han KIM
;
So Ra PARK
;
Sueng Han HAN
;
Jong Bok LEE
Author Information
1. Department of Ophthalmology, Institute of Vision Research, Yonsei University College of Medicine, Seoul, Korea. 491209@yumc.yonsei.ac.kr
- Publication Type:Original Article
- Keywords:
Calcium;
calsequestrin;
inferior oblique overaction;
ryanodine receptor;
sarcoplasmic reticulum;
sarcoplasmic reticulum Ca2+ - ATPase 1
- MeSH:
Sarcoplasmic Reticulum Calcium-Transporting ATPases;
Sarcoplasmic Reticulum/*metabolism;
Ryanodine Receptor Calcium Release Channel/metabolism;
Ryanodine/metabolism;
Oxalates/metabolism;
Oculomotor Muscles;
Ocular Motility Disorders/*metabolism/*pathology;
Muscles/*pathology;
Models, Statistical;
Middle Aged;
Male;
Humans;
Female;
Child, Preschool;
Child;
Calsequestrin/metabolism;
Calcium-Transporting ATPases/metabolism;
Calcium/metabolism/*pharmacokinetics;
Blotting, Western;
Aged;
Adult;
Adolescent
- From:Yonsei Medical Journal
2006;47(2):207-213
- CountryRepublic of Korea
- Language:English
-
Abstract:
We characterized and compared the characteristics of Ca2+ movements through the sarcoplasmic reticulum of inferior oblique muscles in the various conditions including primary inferior oblique overaction (IOOA), secondary IOOA, and controls, so as to further understand the pathogenesis of primary IOOA. Of 15 specimens obtained through inferior oblique myectomy, six were from primary IOOA, 6 from secondary IOOA, and the remaining 3 were controls from enucleated eyes. Ryanodine binding assays were performed, and Ca2+ uptake rates, calsequestrins and SERCA levels were determined. Ryanodine bindings and sarcoplasmic reticulum Ca2+ uptake rates were significantly decreased in primary IOOA (p < 0.05). Western blot analysis conducted to quantify calsequestrins and SERCA, found no significant difference between primary IOOA, secondary IOOA, and the controls. Increased intracellular Ca2+ concentration due to reduced sarcoplasmic reticulum Ca2+ uptake may play a role in primary IOOA.
