Production of Egg Yolk Antibodies Specific to House Dust Mite Proteins.
10.3349/ymj.2014.55.4.999
- Author:
Kyung Eun LEE
1
;
Beom Ku HAN
;
Jae Yong HAN
;
Jung Yeon HONG
;
Mi Na KIM
;
Won Il HEO
;
Myung Hyun SOHN
;
Kyung Won KIM
;
Kyu Earn KIM
Author Information
1. Department of Pediatrics and Institute of Allergy, Brain Korea 21 Plus Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea. kekim@yuhs.ac
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
House dust mite;
immunoglobulin Y;
D. pteronyssinus;
D. farinae
- MeSH:
Allergens/*immunology;
Animals;
Antibodies/*immunology;
Chickens;
Egg Yolk/*immunology;
Female;
Immunoglobulins/*immunology;
Pyroglyphidae/*immunology
- From:Yonsei Medical Journal
2014;55(4):999-1004
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: House dust mites (HDMs) are an important source of indoor allergens associated with asthma, rhinitis and atopic dermatitis. Chicken immunoglobulin (Ig) Y is known to be a good alternative to mice and rabbit antibody production. In this study, we produced IgYs specific to HDMs and investigated their IgE immunoreactivities. MATERIALS AND METHODS: Total IgYs were isolated from the yolks of White Leghorn hens immunized with either Dermatophagoides pteronyssinus or D. farinae protein extract. Control antibodies were separated from the yolks of immunized hens with phosphate buffered saline. IgYs specific to HDMs were analyzed using enzyme-linked immunosorbent assay and Western blotting analysis. RESULTS: The concentration of egg IgY specific to D. farinae in an immunized hen increased and the highest achieved was 661.3 ug/mg (per an egg) on day 47, compared with 760 ug/mg IgY specific to D. pteronyssinus on day 16. The D. pteronyssinus or D. farinae-specific IgY was detected by binding of each mite proteins, and their immunoreactivities were elevated dependent of the specific IgY concentration. CONCLUSION: IgY specific to HDMs may be a promising antibody for immunological diagnosis as well as identification of possible resistance relating to HDM allergy.
