Comparative study on the mechanism and efficacy of Zexie tang traditional decoction and formula granules in reducing lipid accumulation
- VernacularTitle:泽泻汤传统汤剂和配方颗粒降低脂质堆积的作用机制和药效研究
- Author:
Yuanyuan GUO
1
;
Lina MA
2
;
Huqin LIN
1
;
Changhui ZHENG
1
;
Jiayi LI
3
;
Zhijun LI
4
;
Junling CAO
1
Author Information
1. School of Chinese Materia Medica，Beijing University of Chinese Medicine，Beijing 100029，China
2. Dept. of Pharmacy，Dongfang Hospital，Beijing University of Chinese Medicine，Beijing 100078，China
3. Dept. of Pharmacy，Dongzhimen Hospital，Beijing University of Chinese Medicine，Beijing 100700，China
4. Dept. of Pharmacy，Luoyang Hospital，Dongzhimen Hospital，Beijing University of Chinese Medicine，Henan Luoyang 471934，China
- Publication Type:Journal Article
- Keywords:
Zexie tang;
traditional decoction;
formula granules;
non-alcoholic fatty liver disease;
human hepatocellular
- From:
China Pharmacy
2025;36(10):1202-1208
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the effect and mechanism of Zexie tang （ZXT） on reducing lipid accumulation through network pharmacology， and compare the difference of traditional decoction versus formula granules. METHODS The active components and targets of ZXT were identified using TCMSP and SwissTargetPrediction databases. GeneCards， OMIM， DisGeNET and TTD databases were used to analyze the related targets of non-alcoholic fatty liver disease （NAFLD）； protein-protein interaction network model was constructed by String database；“ ZXT-NAFLD target-pathway” network diagram was constructed by using CytoScape software； target enrichment analysis was performed by using Metascape platform. Fat accumulation model of human hepatocellular carcinoma HepG2 cells was established to observe the effects of traditional decoction and formula granules of ZXT on lipid accumulation of cells. RESULTS Alisol B， alisol C， 1-monolinolein and alisol B monoacetate were the key active components of ZXT in the treatment of NAFLD. The core targets included MDM2， MAPK1， PIK3CB， PRKCQ and MAPK14， etc. The core signaling pathways included endocrine resistance， insulin resistance and Th17 cell differentiation. Compared with model group， except for the Zexie formula granules group and Baizhu formula granules group， the absorbance values in all other administration groups were significantly decreased （P＜0.01）； the absorbance value of Baizhu traditional decoction group was significantly higher than that of ZXT traditional decoction group （P＜0.01）； the absorbance values of Zexie formula granule group and Baizhu formula granule group were significantly higher than that of ZXT formula granule group （P＜0.01）； the absorbance value of Zexie formula granule group was significantly higher than that of Zexie traditional decoction group （P＜0.01）； the absorbance value of Baizhu formula granule group was significantly higher than that of Baizhu traditional decoction group （P＜0.01）. CONCLUSIONS ZXT reduces lipid accumulation of human hepatocellular carcinoma cells through multiple components， multiple target and multiple pathways， and its traditional decoction and formula granules exhibit slightly different lipid-lowering effects.
