Non-coding RNAs expression profile of adjacent and distant liver tissues of hepatic cystic echinococcosis lesions
10.16250/j.32.1915.2024216
- VernacularTitle:肝细粒棘球蚴病病灶毗邻及远端肝组织非编码RNA表达谱分析
- Author:
Ibrahim IRSHAT
1
,
2
;
Aikebaier AIZEMAITI
1
,
2
;
Mijiti WUBULIKASIMU
1
,
3
;
Qilin XU
4
;
Abudumijiti ABUDUSIKUER
4
;
Yuanquan WU
4
;
Tuersun KAHAER
1
,
2
Author Information
1. Provincial and Ministerial Co-founded State Key Laboratory of Pathogenesis, Prevention and Treatment of High-Incidence Diseases in Central Asia, Urumqi, Xinjiang 830001, China
2. Department of Hepatobiliary and Pancreatic Surgery, The First People’s Hospital of Kashgar Prefecture, Xinjiang Uygur Autonomous Region, Kashgar, Xinjiang 844000, China
3. Department of Orthopedic Trauma, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830001, China
4. Department of Hepatobiliary and Pancreatic Surgery, The First People’s Hospital of Kashgar Prefecture, Xinjiang Uygur Autonomous Region, Kashgar, Xinjiang 844000, China
- Publication Type:Journal Article
- Keywords:
Cystic echinococcosis;
Liver;
Whole transcriptome sequencing;
Non-coding RNA;
MicroRNA;
Circular RNA;
Long non-coding RNA;
Competing endogenous RNA network
- From:
Chinese Journal of Schistosomiasis Control
2025;37(2):152-162
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the differential expression of non-coding RNAs (ncRNAs) from liver tissues adjacent to hepatic cystic echinococcosis (CE) lesions and distant normal liver tissues using whole transcriptome sequencing, and perform functional annotations of differentially expressed ncRNAs, so as to explore the potential role of ncRNAs in the pathogenesis of CE. Methods Intraoperative liver tissue specimens adjacent to hepatic CE lesions and distant normal liver tissue specimen were sampled from patients with hepatic CE, and the expression profiles of microRNAs (miRNAs), circular RNAs (circRNAs), and long non-coding RNAs (lncRNAs) were detected using whole transcriptome sequencing. Differentially expressed genes were identified, and functional annotations were performed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. In addition, a circRNA/lncRNA-miRNA-messenger RNA (mRNA) competing endogenous RNA (ceRNA) network was constructed using the Cytoscape software, and the expression of hub miRNAs in the network was validated using real-time quantitative reverse transcription PCR (RT-qPCR) assay. Results A total of 41 differentially expressed miRNAs were identified between the adjacent and distal tissues of hepatic CE lesions, including 8 up-regulated and 33 down-regulated miR-NAs, which were significantly enriched in biological processes of Ras signaling and neutrophil activation. Five differentially expressed circRNAs were detected, including 3 up-regulated and 2 down-regulated circRNAs, which were significantly enriched in molecular functions of hormone signaling pathways and RNA transcription regulation. A total of 447 differentially expressed lncRNAs were identified, including 200 up-regulated and 247 down-regulated lncRNAs, which were involved in cell proliferation, immune regulation, and extracellular matrix remodeling pathways. MiRNA target analysis predicted hsa-miR-27a-5p, hsa-miR-21-3p, and hsa-miR-181b-2-3p as hub nodes in the ceRNA network. RT-qPCR assay detected that the relative expression levels of ENSG00000253736, HAS2-AS1, PCSK6, hsa-miR-21-3p, hsa-miR-27a-5p, MIR23AHG, VIPR1-AS1, LINC02910, and hsa-miR-181b-2-3p were 3.00 ± 0.25, 2.75 ± 0.33, 1.01 ± 0.51, 2.65 ± 0.41, 1.01 ± 0.29, 1.10 ± 0.31, 1.05 ± 0.27, 0.25 ± 0.49, and 2.56 ± 0.35 in adjacent tissues of hepatic CE lesions, normalized to that in distant tissues from hepatic CE lesions, respectively (t = 6.21, 5.83, 7.51, 7.46, 6.12, 6.65, 7.13, 1.87 and 7.81, all P values < 0.01), which was consistent with whole transcriptome sequencing results. Conclusions Differentially expressed ncRNAs from adjacent and distal liver tissues of hepatic CE lesions may contribute to the pathological mechanisms of CE through mediating cell proliferation, immune evasion, and inflammatory responses, in which hsa-miR-27a-5p and hsa-miR-21-3p may serve as hub miRNAs.
