Sequencing and analysis of the complete mitochondrial genome of Bulinus globosus
10.16250/j.32.1915.2024262
- VernacularTitle:球形小泡螺线粒体基因组全序列测定及分析
- Author:
Peijun QIAN
1
;
Mutsaka-Makuvaza MASCELINE JENIPHER
2
,
3
;
Chao LÜ
1
;
Yingjun QIAN
1
;
Wenya WANG
1
;
Shenglin CHEN
1
;
Andong XU
1
;
Jingbo XUE
1
;
Jing XU
1
;
Xiaonong ZHOU
1
;
Midzi NICHOLAS
4
;
Shizhu LI
1
,
5
Author Information
1. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), National Health Commission Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai 200025, China
2. Department of Microbiology and Parasitology, College of Medicine and Health Sciences, University of Rwanda, Butare, Rwanda
3. National Institute of Health Research, Ministry of Health and Child Care, Harare, Zimbabwe
4. National Institute of Health Research, Ministry of Health and Child Care, Harare, Zimbabwe
5. School of Global Health, Shanghai Jiao Tong University School of Medicine and Chinese Center for Tropical Diseases Research, Shanghai 200025, China
- Publication Type:Journal Article
- Keywords:
Bulinus globosus;
Mitochondrial genome;
Genome composition;
Phylogeny;
Sequence analysis
- From:
Chinese Journal of Schistosomiasis Control
2025;37(2):116-126
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the structural and phylogenetic characteristics of the mitochondrial genome from Bulinus globosus, so as to provide a theoretical basis for classification and identification of species within the Bulinus genus, and to provide insights into understanding of Bulinus-schistosomes interactions and the mechanisms of parasite transmission. Methods B. globosus samples were collected from the Ruya River basin in Zimbabwe. Mitochondrial DNA was extracted from B. globosus samples and the corresponding libraries were constructed for high-throughput sequencing on the Illumina NovaSeq 6000 platform. After raw sequencing data were subjected to quality control using the fastp software, genome assembly was performed using the A5-miseq and SPAdes tools, and genome annotation was conducted using the MITOS online server. Circular maps and sequence plots of the mitochondrial genome were generated using the CGView and OGDRAW software, and the protein conservation motifs and structures were analyzed using the TBtools software. Base composition and codon usage bias were analyzed and visualized using the software MEGA X and the ggplot2 package in the R software. In addition, a phylogenetic tree was created in the software MEGA X after sequence alignment with the software MAFFT 7, and visualized using the software iTOL. Results The mitochondrial genome of B. globosus was a 13 730 bp double-stranded circular molecule, containing 2 ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, and 13 protein-coding genes, with a marked AT preference. The mitochondrial genome composition of B. globosus was similar to that of other species within the Bulinus genus. Phylogenetic analysis revealed that the complete mitochondrial genome sequence of B. globosus was clustered with B. truncatus, B. nasutus, and B. ugandae into the same evolutionary clade, and gene superfamily analysis showed that the metabolism-related proteins of B. globosus were highly conserved, notably the cytochrome c oxidase family, which showed a significant consistency. Conclusions This is the first whole mitochondrial genome sequencing to decode the compositional features of the mitochondrial genome of B. globosus from Zimbabwe and its evolutionary relationship within the Bulinus genus, which provides important insights for further understanding of the phylogeny and mitochondrial genome characteristics of the Bulinus genus.
