Viral inactivation in von Willebrand factor preparations via UVC irradiation: an experimental approach

Yalu ZHANG; Jie MA; Rong ZHANG; Chunhui YANG

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Viral inactivation in von Willebrand factor preparations via UVC irradiation: an experimental approach

VernacularTitle:短波紫外线灭活血管性血友病因子制品中病毒的研究
Author: Yalu ZHANG ¹ ; Jie MA ² ; Rong ZHANG ¹ ; Chunhui YANG ¹
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1. Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu 610052, China
2. Taibang Biologic Group, Taian 271601, China
Publication Type:Journal Article
Keywords: UVC; vWF; virus inactivation; PPV; PRV; EMCV; VSV
From: Chinese Journal of Blood Transfusion 2025;38(5):673-677
CountryChina
Language:Chinese
Abstract: Objective: To evaluate the efficacy of ultraviolet-C (UVC) irradiation in inactivating porcine parvovirus (PPV), encephalomyocarditis virus (EMCV), pseudorabies virus (PRV) and vesicular stomatitis virus (VSV) within von Willebrand factor (vWF) preparations. Methods: An ultraviolet inactivator (254 nm primary wavelength) was employed to optimize UV exposure dosages for the samples, and the initial test groups were set at five irradiation gradients: 100, 200, 400, 1 000, and 1 500 J/m gradients. Based on the results of the preliminary experiments, subsequent formal experiments implemented refined dosage parameters at 100, 125, 150, 175, and 200 J/m . Virucidal efficacy against various pathogenic strains was evaluated via cytopathic effect (CPE) observation methodology, while simultaneously quantifying von Willebrand factor antigen levels (vWF∶Ag) and collagen-binding activity (vWF∶CBA) using enzyme-linked immunosorbent assays (ELISA) and automated coagulation analysis systems before and after UVC treatment. Results: The results showed that > 100 J/m UVC irradiation achieved a≥4.0 log reduction factor in vWF. For vWF antigen, retention rates were observed to be 93.67%, 91.72%, 93.54%, 79.05%, and 85.50% at UVC doses of 100, 125, 150, 175, and 200 J/m , respectively. Similarly, the retention rates of vWF collagen-binding activity were 81.11%, 87.81%, 86.15%, 73.73%, and 73.72% under the same dose conditions. Conclusion: Considering both inactivation efficacy and functional preservation while ensuring safety and effectiveness, 100-150 J/m represents the optimal inactivation dose.