"Compatibility" Relationship of Active Components and Heat-clearing and Blood-cooling Effect of Rehmannia glutinosa Roots
10.13422/j.cnki.syfjx.20250116
- VernacularTitle:地黄块根中药效成分“配伍”特征及清热凉血功效
- Author:
Yaman CHEN
1
;
Jinpeng CUI
1
;
Juan ZHANG
1
;
Qingpu LIU
1
;
Haiyan GONG
1
;
Jingwei LEI
1
;
Fengqing WANG
2
;
Caixia XIE
1
Author Information
1. Henan Provincial Engineering Technology Research Center for Quality Control and Evaluation of Traditional Chinese Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China
2. College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China
- Publication Type:Journal Article
- Keywords:
Rehmannia glutinosa;
active components;
compatibility;
clearing heat and cooling blood;
relational degree
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2025;31(12):193-201
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo analyze the "compatibility" relationship of sugars and glycosides and the heat-clearing and blood-cooling effect of the roots of four varieties of Rehmannia glutinosa and provide a basis for research on the pharmacodynamic material basis and quality control of R. glutinosa. MethodsThe content of sugars and glycosides in the roots of four varieties of R. glutinosa was determined during the growth period. The principal component analysis (PCA), orthogonal partial least squares-discriminant analysis (OPLS-DA), and the "compatibility" relationship of active components were employed to screen out the differential samples. A rat model of bleeding due to blood heat was used to verify the pharmacodynamic differences and the potential active components of differential samples. ResultsThe content and proportion characteristics of various components in roots of the four varieties of R. glutinosa during the expansion stage and the maturity stage had obvious differences. The proportion of phenylethanoid glycosides at the maturity stage was higher than that at the expansion stage. The R. glutinosa variety 85-5 had special quality characteristics among the tested varieties. All the samples alleviated the symptoms in the rat model. The effect of clearing heat and cooling blood was different between the maturity stage and the expansion stage, as well as between 85-5 samples at the maturity stage and other samples. The effect of clearing heat and cooling blood of R. glutinosa roots was the result of the combined action of multiple components in R. glutinosa roots and might be related to the high proportions of polysaccharides, iridoid glycosides, and phenylethanoid glycosides. ConclusionThe growth stage and variety affect the quality of R. glutinosa roots. The effect of clearing heat and cooling blood of R. glutinosa roots was related to the content and proportions of various components. The study can provide a basis for the basic research on the active components and quality control of R. glutinosa.