Pharmacokinetic Differences of Seven Components in Different Phases of Banxia Xiexintang in Rats
10.13422/j.cnki.syfjx.20250969
- VernacularTitle:半夏泻心汤不同相态中7种成分在大鼠体内的药动学差异
- Author:
Chao HE
1
;
Siyi LIU
1
;
Mingyun WANG
1
;
Qi WANG
1
;
Jingwen ZHOU
1
;
Tong ZHANG
1
;
Yiqiong PU
1
Author Information
1. School of Pharmacy，Shanghai University of Traditional Chinese Medicine，Shanghai 201203，China
- Publication Type:Journal Article
- Keywords:
Banxia Xiexintang;
phase state of decoction;
pharmacokinetics;
ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry（UPLC-QqQ-MS/MS）;
precipitated phase;
colloidal phase
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2025;31(13):215-222
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo evaluate the effects of phases on the pharmacokinetic behavior of seven components from Banxia Xiexintang（BXT） in normal rats by investigating and comparing their pharmacokinetic profiles in different phase samples. MethodsThe phase separation of BXT was carried out by centrifugation-dialysis method， and three phase samples were obtained， including the precipitated phase（PP）， colloidal phase（CP） and true solution phase（TP）. A total of 24 male SD rats were randomly divided into BXT， PP， CP and TP groups（n=6）. The BXT group was gavaged at a dose of 24.1 g·kg-1（calculated by the dosage of raw materials）. After proper treatments， PP， CP and TP groups were administrated at the same dose as that of BXT group， respectively. Blood was collected from each group at set time points after gavage of BXT and the phase samples. The contents of 7 components（baicalin， wogonoside， wogonin， berberine， palmatine， ammonium glycyrrhizinate and isoliquiritin） in rat plasma were determined by ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry（UPLC-QqQ-MS/MS）， and the pharmacokinetic parameters of each component were analyzed by DAS 2.0. ResultsThe peak concentration of baicalin was the highest among the blood-entered components in each group， followed by wogonoside. The results of the concentration-time curves and pharmacokinetic parameters of the 7 components showed that the area under the concentration-time curve（AUC） of isoliquiritin in the BXT group was the highest， followed by that in the CP group. AUC values of baicalin， wogonoside， wogonin and ammonium glycyrrhizinate in the BXT group were similar to those of the CP group， and AUC of palmatine in the BXT group was similar to that of the PP group. The elimination half-life（t1/2） values of baicalin and wogonoside in the BXT group was the longest， the t1/2 values of ammonium glycyrrhizinate and berberine were similar to those of the CP group， and the t1/2 of palmatine was similar to that of the PP group. The t1/2 of wogonin was the longest in the PP group， and the t1/2 of isoliquiritin was the longest in the TP group was the longest， which was similar to that in the PP group. Except for isoliquiritin， the other 6 components showed double peaks in the concentration-time curve of the PP group， indicating that the above components might be reabsorbed through the enterohepatic circulation in vivo， which resulted in the maintenance of high plasma concentrations for a long time， and consequently exhibited sustained-release properties. ConclusionThe pharmacokinetic characteristics of the components in different phases were different， and the CP phase may be the effective phase from the perspective of the pharmacological action of BXT. Compared with the BXT group， the in vivo action times of some components in the CP and PP groups were prolonged. The study explores the phase differences of traditional Chinese medicine（TCM） compound decoction in the aspect of pharmacokinetics， and verifies that the phase states from TCM compound decoction will affect the pharmacokinetic behaviors of the active components， which may consequently lead to the difference in in vivo effects.
