Shaoyaotang Restores Th17/Treg Cell Balance by Regulating Glucose Metabolism Reprogramming in Treatment of Ulcerative Colitis
10.13422/j.cnki.syfjx.20250604
- VernacularTitle:芍药汤通过调控糖代谢重编程影响Th17/Treg细胞平衡治疗溃疡性结肠炎
- Author:
Yiwen WANG
1
;
Yiling XIA
1
;
Erle LIU
1
;
Shaijin JIANG
1
;
Bo ZOU
1
;
Dongsheng WU
1
;
Youwei XIAO
1
;
Hui CAO
1
Author Information
1. The First Hospital of Hunan University of Chinese Medicine，Changsha 410007，China
- Publication Type:Journal Article
- Keywords:
ulcerative colitis;
Shaoyaotang;
glucose metabolism reprogramming;
Th17/Treg cell balance;
2-deoxy-D-glucose （2-DG）;
mouse
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2025;31(13):78-85
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of Shaoyaotang on T helper cell 17/regulatory T lymphocyte（Th17/Treg） cell balance in ulcerative colitis and decipher the intervention mechanism based on glucose metabolism reprogramming. MethodsThe mouse model of ulcerative colitis was established by the dextran sulfate sodium （DSS） method. Forty-eight C57BL/6 mice were randomly allocated into normal， model， Western drug control （mesalazine， 0.39 g·kg-1·d-1）， Shaoyaotang （15.54 g·kg-1·d-1）， inhibitor （2-deoxy-D-glucose， 2-DG， 100 mg·kg-1·d-1）， and inhibitor （2-DG， 100 mg·kg-1·d-1） + Shaoyaotang （15.54 g·kg-1·d-1） groups. Mice were administrated with the corresponding drugs by gavage for 7 days. The general conditions and the colon injury degree were observed 24 h after the last administration. The expression of interleukin （IL）-10 and IL-17 in the colon tissue was detected by immunohistochemical staining. Western blot and Real-time fluorescence quantitative polymerase chain reaction （Real-time PCR） were performed to determine the protein and mRNA levels， respectively， of hypoxia-inducing factor-1α （HIF-1α）， lactate dehydrogenase （LDHA）， and hexokinase 2 （HK2） in the colon tissue. Th17/Treg cell differentiation was detected by flow cytometry. Enzyme-linked immunosorbent assay was employed to measure the levels of lactic acid and glucose in the colon tissue and IL-10， IL-17， and IL-6 in the serum. ResultsCompared with the normal group， the model group showed decreases in body weight and disease activity index （DAI） （P<0.05）， elevations in levels of HIF-1α， LDHA， HK2， IL-17， IL-6， Th17 cells， lactic acid， and glucose in the colon tissue （P<0.05）， and declines in the levels of of IL-10 and Treg cells （P<0.05）. Compared with the model group， the drug administration groups showed increases in body weight and DAI （P<0.05）， declines in levels of HIF-1α， LDHA， HK2， IL-17， IL-6， Th17 cells， lactic acid， and glucose in the colon tissue （P<0.05）， and rises in levels of IL-10 and Treg cells （P<0.05）. Shaoyaotang+2-DG group had the most obvious effect. ConclusionShaoyaotang can relieve diarrhea and bloody stool in mice with ulcerative colitis by restoring the Th17/Treg cell balance via regulation of glucose metabolism reprogramming， thus playing a role in the treatment of ulcerative colitis.
