Mechanism of Exogenous Melatonin in Inhibiting Early Bolting in Angelica sinensis
10.13422/j.cnki.syfjx.20250411
- VernacularTitle:外源褪黑素抑制当归早薹的机制
- Author:
Jiang ZHAO
1
;
Zhanwen TANG
2
;
Tao YANG
1
;
Jie SHA
1
;
Tong PENG
2
;
Weiwen LU
1
;
Yinquan WANG
2
Author Information
1. Key Laboratory of Microbial Resources Exploitation and Application of Gansu Province， Institute of Biology， Gansu Academy of Sciences，Lanzhou 730000，China
2. Gansu University of Traditional Chinese Medicine，Lanzhou 730000，China
- Publication Type:Journal Article
- Keywords:
Angelica sinensis melatonin;
endogenous hormones;
early bolting;
jasmonic acid
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2025;31(13):234-240
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveThis study aims to investigate the effects of different sizes of seedlings and melatonin treatment on physiological and biochemical indicators and bolting-related gene expression in Angelica sinensis， find substances related to early bolting， and elucidate the inhibitory mechanism of melatonin on bolting. MethodsSpectrophotometry was used to detect the related enzyme activities of A. sinensis leaves. The contents of endogenous hormones and polyamines were detected using ultra-high performance liquid chromatography-tandem mass spectrometry. Real-time polymerase chain reaction （Real-time PCR） was used to detect the expression levels of bolting-related genes. Inter-group differential indicator analysis， orthogonal partial least squares discriminant analysis， and principal component analysis were comprehensively applied to identify factors related to early bolting. ResultsEndogenous jasmonic acid and melatonin were identified as the most important factors affecting early bolting. Secondly， the activity of antioxidant enzymes， abscisic acid content， gibberellin content， and the expression levels of CO3， HD3A， and FD genes had important effects on the bolting process. Compared with small seedlings， exogenous melatonin treatment mainly inhibited early bolting by increasing endogenous melatonin content， reducing gibberellin content， and decreasing the expression levels of SOC1 and FD genes. ConclusionExogenous melatonin can inhibit early bolting in A. sinensis by regulating its physiological， biochemical， and gene expression levels.
