Result of antimicrobial activity of antibiotic encapsulated in liposomes
- VernacularTitle: Липосомд суурилуулсан антибиотикийн бичил биетний эсрэг идэвхийг тодорхойлсон дүн
- Author:
Enkhtaivan E
1
,
2
;
Enkhmaa O
1
;
Nyam-Ochir E
1
;
Bayasgalan B
3
;
Odonchimeg M
4
;
Lodoidanzan A
5
;
Unursaikhan S
6
;
Enkhjargal D
3
;
Baatarkhuu O
1
;
Ariunsanaa B
1
Author Information
1. MNUMS
2. Etugen University
3. School of Pharmacy
4. Mongolian Academy of Science, Institute of Chemistry and Chemical Technology
5. Field research center MUST and Nagoya University joint
6. National Center for Public Health
- Publication Type:Journal Article
- Keywords:
phospholipids;
liposomes,nanotechnology;
antibiotic;
MRSA
- From:Mongolian Medical Sciences
2023;205(4):3-8
- CountryMongolia
- Language:Mongolian
-
Abstract:
Introduction:The treatment of antibiotic-resistant bacterial infections has become a pressing problem for humanity
worldwide, and antibiotic-resistant bacterial infections are likely to be the leading cause of death
by 2050.Due to the mutation of infectious disease-causing bacteria and the emergence of bacterial
resistance due to the improper use of antibiotics, the time and cost of infectious disease treatment
increases, and in some cases, it leads to an increase in mortality, so it is the focus of the health sector
in every country, regardless of the income level of the population. In addition, bacterial resistance has
a negative impact on public health, food safety, the environment, and the economy.
As of 2015, Mongolia ranks among the countries with the highest consumption of antibiotics in the
world, with 64.41 units of antibiotics prescribed per 1,000 people per day. Bacteria resistant to broad
spectrum antibiotics have increased dramatically, and among Gram-positive bacteria, drug-resistant
Staphylococcus aureus (MRSA) has become one of the most common and dangerous cause
Purpose:Determine the external structure of liposome-encapsulated antibiotics and evaluate their antibacterial
activity.
Materials and Methods:We conducted this study using an experimental research design. Phospholipids were isolated by
intermittent evaporation, antibiotic encapsulation by freeze-thaw method, and antibiotic sensitivity was
determined using standard strains by disc diffusion andmicro dilution method.
Research ethics:Permission to submit the survey was granted by the Ethics Review Committee of the MNUMS. The
survey was granted in accordance with the rules and regulations.
Results:In liposome-encapsulated antibiotic sensitivity assays, azithromycin and clarithromycin did not form
sacred circles, whereas doxycycline hyclate was sensitive by forming a 16 mm circle. Doxycycline
hyclate encapsulated in liposomes formed a 16 mm circle with sensitive results, whereas blank liposomes were inactive. When the rabbits were infected with a standard strain of methicillin-resistant
Staphylococcus aureus, the infected area was purulent 24 hours later. A cream containing antibiotics
was started at this time. A deep wound was recovered after 12 days after the pus was removed.
Nevertheless, after 24 days, the wound on the rabbit’s infected part healed and the hair on the scraped
part grew back.
Conclusion:According to the dilution method, liposome-encapsulated doxycycline hyclate inhibited bacterial
growth at 2-fold lower doses than pure doxycycline hyclate. In experimental animal models,
liposome-based antibiotic ointment has shown antibacterial activity.
- Full text:2025060517444735021MMS-2023-205(4)-3-8.pdf
