Some pharmacological study results of Lish-6
- VernacularTitle:Уламжлалт Лиш-6 эмийн фармакологийн зарим үр дүн
- Author:
Enkhtungalag S
1
,
2
;
Dejidmaa B
3
;
Odonchimeg Ch
3
;
Tsend-Ayush D
4
;
Chimedragchaa Ch
3
;
Erdenechimeg Ch
3
Author Information
1. Department of Traditional Prescriptionology, International School of Mongolian Medicine, MNUMS
2. School of Medicine, MNU
3. Institute of Traditional Medicine and Technology
4. Innovation and Prescription Research Section, Institute of Mongolian traditional Medicine, MNUMS
- Publication Type:Journal Article
- Keywords:
Traditional drug;
Pain;
LD50;
Acetic acid induced pain;
Bacteria
- From:
Mongolian Journal of Health Sciences
2025;86(2):138-142
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:Lish-6 has been used for treatment pharyngitis, flu and throat disease. Lish-6 is composed from Eugenia
caryophylla Thumb, Saussurea lappa C.B.Clark, Schizostachoum chinense Rendle, Glycyrrhiza uralensis Fisch, Gentiana
algida Pall, Terminalia chebula Retz. Anti-fever properties of these plants and their bio-active compounds have
extensively been studied.
Aim:To determine the pain relief and antibacterial effects of Lish-6.
Materials and Methods:To conduct acute toxicity study using V.B. Prozorovsk method. Average lethal dose, lethal and
maximum nonlethal doses were determined. Acetic acid (1%-0.1 ml) was injected into the rat abdominal cavity to induce
pain. Wistar rat of either sex (n = 6) weighing 18–22g were used. All animals were withdrawn from food 2h before the start
of experiment and were divided in five groups. Group I was injected with normal saline (10ml/kg) as control, Diclodenk
was given orally at concentration of 25 mg/kg. Group II III, IV, V and VI were injected with Lish-6 was given orally at
concentrations of 9.2, 18, 36, 92, 184 mg/kg injection of acetic acid. The number of abdominal constrictions (writhes)
were counted of acetic acid injection for the period of 20 min. To determine the antibacterial effect by dilution method.
Results:Average lethal dose of Lish-6 was found to be LD50=0.92 (0.6-1.04) g/kg suggesting that it is slightly toxic animals.
Control group animals abdominal constrictions 72.4±8.8. Lish-6 concentrations of 9.2, 18, 36, 92, 184 mg/kg group
animal reduced acitic acid induced pain by 41.9-78.7% suggesting that it is pain relief effect (p<0.001). 4 g of Lish-6
medicine is active against gram-positive bacteria (S.aureus, Streptococcus pneumonia). However, it is inactive against
gram-negative bacteria. In other words, Lish-6 medicine inhibits the growth of methicillin-resistant and non-resistant
S.aureus bacteria at a dose of 500 mg. It was also found to inhibit Streptococcus pneumonia at a dose of 250 mg.
Conclusion:Average lethal dose of Lish-6 was found to be LD50=0.92 (0.6-1.04) g/kg suggesting that it is slightly toxic
animal. Lish-6 reduced acitic acid induced pain by 41.9-78.7% suggesting that it is pain relief effect. Lish-6 traditional
drug has an antibacterial effect.
- Full text:2025052913101708219138-142.pdf
