The inhibitory effect of curcumin on cell proliferation in cisplatin-resistant cervical cancer cells
- VernacularTitle:Цисплатинд тэсвэртэй умайн хүзүүний хорт хавдрын эсийн өсгөврийг саатуулах куркумины нөлөө
- Author:
Huang Dandan
1
,
2
;
Erdenezaya O
3
;
Damdindorj B
4
;
Adilsaikhan M
5
;
Bolorchimeg B
1
Author Information
1. Departments of Obstetrics and gynecology, School of Medicine, MNUMS
2. Inner Mongolia Baogang Hospital
3. Department of Anatomy, SBM, Institute of Biomedical Sciences, MNUMS
4. Mongolian National University of Medical Sciences
5. Mongolia Japan Hospital, MNUMS
- Publication Type:Journal Article
- Keywords:
Cervical cancer;
Hela/DDP;
SiHa/DDP;
Drug resistance;
Chemotherapy;
Colony formation assay
- From:
Mongolian Journal of Health Sciences
2025;85(1):196-200
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:Cervical cancer is a common disease among women. Treatment for cervical cancer includes surgery, radiation therapy, chemotherapy, or a combination of chemotherapy and radiation therapy. Cisplatin is the first-line chemotherapy drug for cervical cancer. Research has shown that about 20% of cervical cancer patients become resistant
to chemotherapy, which results in decreased results, tumor recurrence, and poor prognosis. Therefore, researching new
drugs, improving the sensitivity of cervical cancer cells to cisplatin, and improving the effectiveness of cervical cancer
treatment is the basis of this research.
Aim:To investigate the inhibitory effect of curcumin on cisplatin-resistant cervical cancer Hela/DDP and SiHa/DDP cell
lines.
Materials and Methods:The study utilized cisplatin-resistant cervical squamous carcinoma (SiHa/DDP) and adenocarcinoma (Hela/DDP) cell lines. The cells in the experimental group were treated with 8.5 μM of curcumin, while the
control group received only the culture medium. A colony formation assay was conducted to assess cell proliferation, with
colonies stained using crystal violet; the number of colonies was then counted and compared between the two groups.
Results : 1. In the Hela/DDP cell line, the control group formed an average of 507.7±15.70 colonies, whereas the experimental group, treated with curcumin, formed 112.3±16.17 colonies. The difference between the groups was statistically
significant (p < 0.0001). 2. In the SiHa/DDP cell line, the control group had an average of 450.3±17.95 colonies, while
the experimental group treated with curcumin had 198.3±13.05 colonies. This difference was also statistically significant
(p < 0.0001).
Conclusions:1. Curcumin significantly reduces the proliferation of cisplatin-resistant cervical squamous cell carcinoma (Hela/DDP)
cells.
2. Curcumin significantly reduces the proliferation of cisplatin-resistant cervical adenocarcinoma (SiHa/DDP) cells.
- Full text:2025052716312661223196-200.pdf
