Study of the subacute toxicity and antibacterial activity of Phythonephro-SAN Preparation
- VernacularTitle:Фитонефро-САН бэлдмэлийн архагдуу хорон чанар болон бактерийн эсрэг үйлдлийн судалгаа
- Author:
Batdavaa B
1
,
2
;
Maral-Erdene G
1
,
3
;
Chimgee Ts
1
;
Enkhmaa G
1
;
Yeruult Ch
1
Author Information
1. Department of pharmacology, School of biomedicine, MNUMS
2. Department of forensic medicine, NFA
3. National center for pathology
- Publication Type:Journal Article
- Keywords:
OECD-407;
Broth dilution method;
Subacute toxicity;
Antibacterial activity
- From:
Mongolian Journal of Health Sciences
2025;85(1):173-178
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:Urinary tract infections (UTIs) are common, affecting 150 million people worldwide annually. It is estimated that 1% of the population suffers from urinary tract infections. The most common infections in kidney and urinary tract
are Escherichia coli, Staphylococcus saprophyticus, Klebsiella, Enterobacter and Proteus which account 80%, 5-15%
and 5-10%, respectively. Oxidative stress, inflammation, and apoptosis are critical factors involved in the pathogenesis
of kidney disease. Oxidative stress, a pathological condition characterized by an imbalance between reactive oxygen species (ROS) and the body’s antioxidant defenses, leads to cellular damage and is directly implicated in the initiation and
progression of acute kidney injury. Antioxidants serve a protective role by mitigating the harmful effects of free radicals
and oxidative stress on cellular structures. Drawing upon the extensive resources of medicinal plants and the therapeutic
practices of traditional medicine, plants rich in antioxidant compounds, including Dasiphora fruticosa (L.), Cynara scolymus (L.), and Rosa acicularis (L.), were selected for the development of the Phytonephro-SAN preparation. The phytochemical profile and nephroprotective properties of these plants have been investigated and validated. Moving forward,
further studies are warranted to assess the safety profile of the formulation, including comprehensive toxicity evaluations.
Aim: To investigate and establish the subacute toxicity and antibacterial activity of the Phytonephro-SAN preparation.
Materials and Methods:The subacute toxicity assessment of the Phythonephro-SAN preparation was conducted on
Wistar rats following the OECD-407 guidelines. The study of the antibacterial activity of the preparation was determined
by the broth dilution method.
Results:The subacute toxicity assessment, evaluated through parameters such as body and organ weights and complete blood count (CBC), revealed no statistically significant differences between the groups administered the Phytonephro-SAN preparation at doses of 500 mg/kg and 1000 mg/kg, and the control group. According to the study of antibacterial activity, Phytonephro-SAN preparation has antibacterial activity at 90 mg/ml and 80 mg/ml doses.
Conclusion: The administration of the Phytonephro-SAN preparation to Wistar rats at doses of 500 mg/kg and 1000
mg/kg over 28 days did not result in mortality, and no significant changes were observed in body and organ weights or
CBC parameters. These findings support the conclusion that the preparation possesses minimal toxicity. Additionally, the
preparation demonstrated effective antibacterial activity against specific urinary tract pathogens at higher concentrations.
- Full text:2025052716125195428173-178.pdf
