Subchronic toxicity and sedative activity studies of herbal complex preparations
- VernacularTitle:Ургамлын гаралтай нийлмэл бэлдмэлийн архагдуу хорон чанар болон тайвшруулах үйлдлийн судалгаа
- Author:
Maral-Erdene G
1
,
2
;
Batdavaa B
1
,
3
;
Chimgee Ts
1
;
Yeruult Ch
1
;
Enkhmaa G
1
Author Information
1. Department of Pharmacology, School of Biomedicine, MNUMS
2. National Center for Pathology
3. Department of Forensic Medicine, NFAM
- Publication Type:Journal Article
- Keywords:
Valeriana officinalis L.;
Leonurus sibiricus L.;
Biochemical parameters;
Histopathology;
Hole-board test
- From:
Mongolian Journal of Health Sciences
2025;85(1):168-172
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:According to the World Health Organization (WHO), approximately 70-95% of developing countries rely
on traditional medicine, which includes around 365 plant, animal, and mineral-based preparations. Natural products consist of numerous biologically active compounds that exert effects against pathogens, making up about 25% of modern
pharmaceuticals derived from plants. Since plants are a combination of various metabolites, they can have therapeutic
effects, side effects, and toxicity in the human body. Based on the traditional use of medicinal herbs in Mongolian and
Tibetan medicine for their sedative properties, we selected the medicinal herbs Valeriana officinalis L. and Leonurus sibiricus L., The objective is to develop new medicinal preparations that can be utilized in modern medical practice to treat,
prevent, or supplement the treatment of depression and anxiety. Consequently, it is necessary to prepare a herbal complex
from these selected plants and conduct studies to investigate their subchronic toxicity and sedative activities.
Aim:To study subchronic and sedative activity of herbal complex preparations.
Materials and Methods:The herbal complex preparation was prepared from the 70% ethanol extract of the roots of Valeriana officinalis L. and the aerial parts of Leonurus sibiricus L., and a compound preparation was made in a 30:70 ratio.
Subchronic toxicity study was conducted on Wistar rats weighing 180-250 g according to the OECD-407 guidelines. The
sedative activity of herbal complex preparation was studied on C57BL/6 and BALB/c mice using the dark/light transition
test according to Takao K., and the hole-board test according to Hiroshi Takeda.
Results: In the sub-chronic toxicity study of the herbal complex preparation, biochemical analysis of the serum (including
ALT, AST, creatinine, and urea) and histopathological examination of the liver, kidney, and heart showed no statistically
significant changes when comparing the experimental groups to the control group. The herbal complex preparation at a
dose of 1000 mg/kg increased the time spent in the dark area, decreased the time spent in the light area, and the number
of transitions between the two areas of mice in the dark/light transition test, and reduced the number of head-dipping into
the holes of mice in the hole-board test.
Conclusion: The herbal complex preparation exhibited low toxicity at doses of 1000 mg/kg and 1500 mg/kg based on
biochemical and histopathological examinations in the subchronic toxicity study. Furthermore, the preparation demonstrated sedative effects at a dose of 1000 mg/kg.
- Full text:2025052716090424663168-172.pdf
