Study on the histochemical investigation of the esophageal endoscopic biopsies
- VernacularTitle:Улаан хоолойн уян дурангийн амьд сорьцын шинжилгээг хистохимийн аргаар судалсан дүн
- Author:
Munguntsetseg S
1
,
2
;
Bayarmaa E
1
,
3
;
Adilzaya D
1
,
3
Author Information
1. Department of Pathology and Forensic Medicine, School of Biomedicine, MNUMS
2. National Center of Pathology
3. Department of Pathology, Mongolia-Japan Hospital, MNUMS
- Publication Type:Journal Article
- Keywords:
sophageal cancer;
Precancerous lesions;
Gastroesophageal reflux disease;
Barrett’s esophagus
- From:
Mongolian Journal of Health Sciences
2025;85(1):155-158
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background: Gastroesophageal reflux disease (GERD) composes up to 28% of the esophageal disorders, and diagnosis
of GERD is associated with a 10-15% of risk of Barrett’s esophagus, change of normal squamous epithelium of the distal
esophagus to a columnar-lined intestinal metaplasia. It is reported that gastrointestinal (GI) disorders are the second out of
five leading diseases in ambulatory diagnosed diseases and in 2022, esophageal adenocarcinoma is ranked in the fourth of
the 10 most prevalent cancers among Mongolian population. When the Barrett’s esophagus changes shift to dysplasia, risk
of esophageal adenocarcinoma development rises 30-125 folds and every year, 0.8% of population with dysplastic changes suffer from esophageal carcinoma. Although specific staining methods for histochemical analysis has been introduced
into pathological laboratories detecting protein, fat, nucleic acid and enzymes that contained in cells, there isn’t study has
been conducted to investigate the diagnostic specificity of Alcian blue and PAS staining method among population with
Barrett’s esophagus.
Aim:We aimed to make a differential diagnosis with basic and histochemical staining method in esophageal biopsies
from Second Central Hospital of Mongolia (SCHM) and Mongolia-Japan hospital of MNUMS.
Results:A total of 589 biopsies were collected from the SCHM and Mongolia-Japan Hospital of MNUMS and hematoxylin and eosin-stained results were Barret’s esophagus (43 biopsies or 7.3%), followed by Squamous cell carcinoma
(46 or 7.8%), GERD (60 or 10.2%), hyperplastic polyp (92 or 15.6%), chronic erosive lesions (242 or 41%), polyp (94
or 15.9%), adenocarcinoma (12 or 2%), respectively. We have re-examined 43 cases with Barrett’s esophagus staining
with Alcian blue and PAS and as a result, acidic mucin will be blue, neutral mucin will be purple, mixed mucin will be
blue-purple and nucleus will be stained blue, respectively. Immunohistochemical re-staining of 43 biopsies with BE, Alcian blue were 87.8% and PAS were 97.4%.
Conclusion: Barrett›s esophagus, esophageal papilloma and hyperplastic polyps are comprising most of the esophageal
precancerous disorders, and 9.8% of esophageal carcinoma was diagnosed. Histochemical re-staining of esophageal intestinal metaplastic changes is significantly beneficial to confirmation of the diagnosis.
- Full text:2025052715534980553155-158.pdf
