Results of comparative study of liposome formation methods
- VernacularTitle:Липосом үүсгэх аргуудыг харьцуулсан судалгааны дүн
- Author:
Khaliun J
1
;
Ariungerel T
1
;
Buyankhishig D
1
;
Jambaninj D
1
Author Information
1. Department of Pharmaceutical Technology, School of Pharmacy, MNUMS
- Publication Type:Journal Article
- Keywords:
Thin film hydration;
Ethanol injection;
Heating method;
Concentration;
Spectrophotometer
- From:
Mongolian Journal of Health Sciences
2025;85(1):57-61
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:Liposomes have been widely studied in the field of medicine in recent years for they can reduce side effects
and regulate drug release by delivering active ingredients to target tissues and cells through active or passive routes. There
are many types of methods for formulating liposomes, and comparing those methods and choosing the most suitable
one will allow the delivery of active ingredients and their incorporation into pharmaceutical forms, so this research was
carried out.
Aim:To compare liposome formation methods.
Materials and Methods: The research work was carried out with the support of “Drug Design Laboratory” and “Pharmaceutical Analysis Laboratory” of the School of Pharmacy of MNUMS. To formulate liposomes, 5 different concentrations
of samples were prepared using thin-film hydration, ethanol injection, and heating methods. Phospholipid concentration
and light absorption were determined by spectrophotometer in each sample. The size of the resulting liposome was determined using a Nanophox.
Results:Phosphatidilcholines were dissolved in distilled water at concentrations of 5, 10, 20, 30, 40, and 50 μg/ml and a
standard curve was established by spectrophotometry to determine the concentration of phospholipids in liposome samples. The highest formulation was 76.98% when 75 mg of phospholipid was used in the preparation by thin film hydration
method, when 50 mg of phospholipid was used in the preparation by ethanol injection method, the highest formulation
was 85.17%, and for when 50 mg of phospholipid was used in the preparation by heating method, the highest formulation
was 58.45%. The mean liposome size by ethanol injection method was 115 nm.
Conclusions:1. Ethanol injection method for liposome formulation is more efficient compared to thin-film hydration method and
heating method.
2. Liposome size was 115 nm when prepared by ethanol injection.
- Full text:202505271357006919757-61.pdf
