Adsorption properties of amorphous aluminum hydroxyphosphate sulfate adjuvant and aluminum hydroxide adjuvant for recombinant hepatitis B surface antigen
10.13200/j.cnki.cjb.004491
- VernacularTitle:无定形羟基磷酸硫酸铝佐剂和氢氧化铝佐剂对重组乙型肝炎表面抗原的吸附特性分析
- Author:
JIANG Ying
- Publication Type:Journal Article
- Keywords:
Aluminum adjuvant;
Hepatitis B surface antigen(HBsAg);
Ligand exchange;
Adsorption rate;
Adsorption strength
- From:
Chinese Journal of Biologicals
2025;38(05):513-520
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the adsorption mechanism of amorphous aluminum hydroxyphosphate sulfate(AAHS)adjuvant and aluminum hydroxide adjuvant on recombinant hepatitis B surface antigen(HBsAg) and the effect of differentadsorption rates on the titer of hepatitis B.MethodsThe Langmuir adsorption equation was used to calculate the maximumadsorption capacity of HBsAg on the surface of AAHS and aluminum hydroxide adjuvants at different concentrations ofsodium chloride(NaCl) and ethylene glycol. Combined with the influence of different concentrations of phosphate on theadsorption effect of HBsAg, the main forces affecting the adsorption of HBsAg and two aluminum adjuvants were analyzed,and the difference between the two adjuvants was also compared. ED_(50)was used to evaluate the immune effect of sampleswith different phosphate concentrations, and the relationship between different adsorption rates and titers was analyzed.ResultsDifferent concentrations of NaCl and ethylene glycol did not affect the adsorption rate of HBsAg, and the adsorptionrate decreased with the increase of phosphate concentration. The titer of samples with lower adsorption rate was still withinthe qualified standard range.ConclusionHBsAg is mainly adsorbed on the surface of AAHS and aluminum hydroxide adju-vants through ligand exchange, and hepatitis B titer may not require high antigen adsorption rate in vitro.
