Results of a study of changes in pancreatic tissue structure in alloxan-induced diabetic mice
- VernacularTitle:Аллоксаны чихрийн шижинтэй хулганы нойр булчирхайн эдийн бүтцийг судалсан дүн
- Author:
Nyamsurendejid D
1
;
Dolgorsuren A
1
;
Amgalanbaatar D
1
;
Avirmed A
1
Author Information
1. Department of Anatomy, School of Biomedicine, MNUMS
- Publication Type:Journal Article
- Keywords:
diabetes, alloxan, experimental animal
- From:
Mongolian Journal of Health Sciences
2025;87(3):147-152
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:Alloxan is a chemical compound commonly used in experimental
animal models of diabetes. In 1943, Shaw Dunn and Mc-
Letchie reported that alloxan causes specific necrosis of pancreatic
β-cells in experimental animals, which led to the study of alloxan in
relation to diabetes. In this study, we evaluated the structural changes
in the pancreatic tissue of diabetic mice induced by alloxan.
Aim:To study the structural changes in the pancreatic tissue of diabetic
mice induced by alloxan.
Materials and Methods:According to the protocol for establishing a
diabetic model, alloxan monohydrate was injected intraperitoneally into
6- to 8-week-old C57BL/6 mice to develop a diabetic model and evaluate
the structure of pancreatic tissue.
Results:Alloxan induced severe degenerative changes in the centers
of the pancreatic islets of mice in the diabetic group. The islet shape
was irregular, and the central part was relatively sparse; dead (karyolysis)
cells were observed. In the exocrine part of the pancreas, the acinar
structure was preserved; the nuclei of acinar cells were stained
bright blue. The plasma-particle ratio of some acinar cells was lost, the
plasma contained vacuoles, and the interstitial spaces were enlarged
and appeared pale. On the 7th day of the experiment, the positive expression
of β-cells in the pancreatic islets of mice in the diabetic group
was reduced compared to the control group, and a necrotic area was
observed. On the 28th day, the positive expression of β-cells was visible
in the central part of the islet. When the qualitative characteristics
of the positively stained cells of the islets of Langerhans were converted
into quantitative values, the percentage of the area of insulin-positive
stained cells was 13.37% ± 0.89% in the control group and 6.01%
± 0.39% in the diabetic group. The percentage of glucagon-positive
stained cells in the control group was 15.27% ± 1.11%, and in the diabetic
group was 5.01% ± 0.58%. The islet area of the pancreatic islets
of Langerhans in the diabetic group was observed to be increased
compared to the control group. This is thought to be due to the swelling
of the islet cells and the formation of empty spaces created by necrotic
cells.
Conclusion:The results of the functional assay showed that glucose-
dependent insulin secretion was still active after 28 days of the
experiment. Alloxan-induced necrosis and apoptosis reduced the percentage
of insulin- and glucagon-positive cells in the islets of Langerhans.
- Full text:2025052421192269266147-153.pdf
