Changes in rat behavior, lung, and hippocampal effects due to vaping exposure
- VernacularTitle:Электрон тамхины нөлөөгөөр туршилтын амьтны зан төрх, уушги, гиппокампын бүтцэд гарах эд ба эсийн өөрчлөлтүүдийг судалсан дүн
- Author:
Oyungerel S
1
;
Tumenbayar B
1
;
Javzandulam B
1
;
Chimegsaikhan S
1
;
Khulan B
1
;
Altantsetseg B
1
;
Mandakh-erdene B
1
;
Munkhsoyol E
2
Author Information
1. Department of Histology, School of Biomedicine, MNUMS
2. Department of Physiology, School of Biomedicine, MNUMS
- Publication Type:Journal Article
- Keywords:
Rat, Dry hit, Nicotine, Bronchoalveolar lavage, Lung
- From:
Mongolian Journal of Health Sciences
2025;87(3):141-146
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:Nicotine-containing vaporized liquids—composed of
propylene glycol, glycerin, water, flavorings, and the thickening agent
vitamin E acetate—have been implicated in the development of EVALI.
Under conditions of low liquid levels and overheating, these substances
release toxic carbonyl compounds (e.g., formaldehyde, acetaldehyde,
acrolein). In a 2019 national survey, 3.5% of adolescents aged 13–15
reported cigarette use, and 10% used e-cigarettes.
Aim:To study the changes in the behavior, lung, and hippocampal
structures of rats due to the effects of electronic cigarettes and their
vapor.
Materials and Methods:In this study, nineteen 14-day-old SHR rats
were exposed to 1 g/mg/day of nicotine and dry hit vapor (control, nicotine
and dry hit groups) for four weeks. Behavioral assessments (Open
Field Test, Elevated Plus Maze, Conditioned Place Preference), bronchoalveolar
lavage (BAL), and histological analysis of lung and hippocampal
tissue were conducted.
Results:The dry hit vapor group showed significantly reduced body
weight (p=0.034), increased anxiety (p=0.006, p=0.025), and avoidance
of the smoky chamber. BAL revealed elevated total cells, neutrophils,
and macrophages (p=0.01, p=0.04) in both nicotine and dry hit groups.
Lung tissue exhibited alveolar septal thickening, inflammation, and emphysema-
like changes. Extensive neuronal death was observed in the
hippocampus.
Conclusion:Anxiety-like behavior was observed in both the burn and
control groups. Analysis of BAL in the dry hit group revealed inflammation
predominantly characterized by macrophage infiltration. Histological
examination of lung tissue from both experimental groups
demonstrated a reduction in the number of alveoli, accompanied by
acute inflammation and thickening of the interalveolar septa. In the hippocampal
region, neuronal loss and a reduction in neuronal density
were also observed.
- Full text:2025052421072354859141-146.pdf
