Impact of Spermatogenic Activity on the Outcome of Sperm Retrieval Procedures in Azoospermic Patient
- VernacularTitle:Азооспермийн үед төмсөгний сперматогенезыг үнэлж, эр бэлгийн эс авах мэс ажилбарын үр дүнтэй харьцуулан судалсан дүн
- Author:
Myagmarsuren P
1
,
2
;
Narantsog Ch
3
;
Sayamaa L
3
;
Javkhlantugs D
1
;
Bayarmaa E
3
;
Bayan-Undur D
1
;
Munkhzol M
2
;
Odkhuu E
2
Author Information
1. Center of Urology Andrology, FCHM
2. Department of Pathophysiology, School of Biomedicine, MNUMS
3. Department of Pathology and Forensic Medicine, School of Biomedicine, MNUMS
- Publication Type:Journal Article
- Keywords:
Azoospermia, Sperm retrieval, Johnson’s score, TEX101, M-TESE
- From:
Mongolian Journal of Health Sciences
2025;87(3):136-140
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:Azoospermia is the most severe form of male infertility,
affecting approximately 1% of male population and 10–15% of infertile
men. In azoospermia cases, sperm retrieval from the testis or epididymis
through surgical procedures is used for assisted reproductive treatments.
When no sperm is retrieved, recent approaches in medicine
suggest using immunohistochemical methods to evaluate spermatogenesis
in testicular tissue and plan further treatments.
Aim:To evaluate spermatogenesis in testicular tissue of azoospermic
patients using immunohistochemistry and compare the findings with
the clinical outcomes of sperm retrieval procedures.
Materials and Methods:This study included 71 azoospermic men who
underwent micro-TESE procedures at the IVF center (RMC) between
2019 and 2023. The excised testicular tissues were fixed, processed
histologically, and evaluated using Johnson’s score. The presence of
spermatozoa in seminiferous tubules was detected by immunohistochemical
and immunofluorescence staining, using markers such as
TEX101 and LDHC.
Results:Johnson’s score was categorized into three groups: poor,
moderate, and good spermatogenesis. These were statistically compared
with hormonal levels and surgical sperm retrieval outcomes.
There were significant differences in Johnson’s scores and serum FSH
and LH levels among the three groups (p < 0.005). TEX101 and LDHC
proteins were strongly expressed in the good group, weakly in the moderate
group, and absent in the poor group. The success rate of sperm
retrieval was 100% (17/17) in the good group, 96.29% (26/27) in the
moderate group, and only 29.62% (8/27) in the poor group.
Conclusion:Histological evaluation of spermatogenesis in azoospermic
patients can help predict the outcome of surgical sperm retrieval,
indicating its clinical value in treatment planning.
- Full text:202505242053562235136-140.pdf
