The Effect of “Hot Natured 3 Herbs” in a Tumor-Induced Model in C57BL/6 Mice
- VernacularTitle:C57BL/6 хулганад үүсгэсэн хавдрын эмгэг загварт “Өвсөн гурван халуун”-ы нөлөөг судалсан дүн
- Author:
Narankhuu R
1
;
Gansukh Ch
2
;
Enkhsaikhan L
2
;
Uranbileg B
3
;
Enkhmaa D
3
;
Odontuya G
4
;
Chimgee Ts
5
;
Ulziisaikhan B
2
;
Enkhtushig G
2
;
Sarantuya L
2
;
Suvd O
2
;
Amgalan B
2
;
Sanjpurev D
6
;
Tserendagva D
7
;
Tsend-Ayush D
1
;
Tserentsoo B
1
Author Information
1. Department of Traditional Prescriptionology, ISMM, MNUMS
2. Department of Microbiology and Immunology, School of Pharmacy and Biomedicine, MNUMS
3. Department of Medical Chemistry, School of Bio-Medicine, MNUMS
4. Basic Research Laboratory, Mongolian University of Pharmaceutical Sciences
5. Department of Pharmacology, School of Bio-Medicine, MNUMS
6. Department of Medicinal Chemistry and Botany, School of Pharmacy, MNUMS
7. MNUMS
- Publication Type:Journal Article
- Keywords:
Hot Natured 3 Herbs, tumor model
- From:
Mongolian Journal of Health Sciences
2025;87(3):44-53
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:Cancer incidence and mortality are steadily increasing both
globally and in Mongolia. As these rates rise, traditional Mongolian medicine
has long utilized herbal formulas for the treatment of gastric and esophageal
cancers and precancerous conditions. One such formulation—Hot Natured
3 Herbs (HN3H)—comprises three species from the Ranunculaceae family:
Atragene sibirica L., Ranunculus repens L., and Pulsatilla bungeana L.. However,
scientific validation of its anti-tumor effects is essential. This study aimed
to investigate the effect of HN3H in a tumor-induced animal model.
Aim:To identify the biologically active compounds of HN3H and evaluate their
effect in an experimentally induced tumor model in animals.
Materials and Methods:The three herbs comprising HN3H—Atragene sibirica
L., Ranunculus repens L., and Pulsatilla bungeana L.—were collected
during their flowering stage (May–June) in Khishig-Undur, Bulgan province,
and dried according to official procedures. Extraction was carried out by maceration
in 96% ethanol at a 1:10 ratio. The concentrated extract was suspended
in water (1:1) and successively fractionated with dichloromethane, ethyl
acetate, butanol, chloroform, and n-hexane. The study was approved by the
Research Ethics Committee of the Mongolian National University of Medical
Sciences (Protocol №2020/03-04). A colorectal cancer model was established
by subcutaneous injection of MC-38 cells (Kerafast, USA) into C57BL/6 mice.
Immunohistochemistry was performed using CK20, CDX2, Ki67, and p53 antibodies
at 1:100 and 1:200 dilutions.
Results:The ethanol extract of HN3H contained 2.98±0.04% total phenolics
and 2.16±0.05% total flavonoids. Body weight and tumor volume were
measured daily with three repetitions. All groups showed a time-dependent
increase in body weight. Mice in groups 1A and 1B received ethanol extract at
50 and 100 mg/kg doses; groups 2A and 2B received dichloromethane extract
at the same doses. The negative control group was administered 0.5 mg/kg
PBS orally, while the positive control group received intraperitoneal injections
of 5-fluorouracil (5FU) at 10 mg/kg twice a week. Tumor growth increased in a
time-dependent manner across groups. Compared to the negative control, tumor
volumes in four treatment groups showed statistically significant reduction
(p˂0.05), while no significant difference was observed when compared to the
positive control (p=0.08). Histological analysis revealed necrosis in all groups,
with variation in extent.
Conclusion:The ethanol extract of HN3H exhibited moderate levels of phenolic
compounds and a high concentration of flavonoids. HN3H extract inhibited
tumor progression and activated lymphocyte-predominant inflammation in
tumor tissues, indicating potential anti-tumor activity (p˂0.05).
- Full text:202505232212257098944-53.pdf
