The study result of the effect of Jirug-6 suppositories on vaginal candidiasis model in experimental animals
- VernacularTitle:Туршилтын амьтанд үүсгэсэн үтрээний кандидозын эмгэг загварт Жирүг-6 лаа эмийн нөлөөг судалсан дүн
- Author:
Yilina
1
,
2
;
Nomin-Erdene U
3
;
Enkhjargal D
1
Author Information
1. Department of Pharmaceutical chemistry and Pharmacognosy, School of Pharmacy, MNUMS
2. School of Mongolian Medicine, Inner Mongolia Medical University, P.R.China
3. Department of Acupuncture and Moxibustion, ISMM, MNUMS
- Publication Type:Other Types
- Keywords:
Vaginal inflammatory;
Jirug-6 suppositories;
Candica albicans
- From:
Mongolian Journal of Health Sciences
2025;88(4):82-87
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:Vaginal candidiasis is a highly prevalent infectious disease among women caused by a wide range of patho
genic organisms. Although it can be treated with standard pharmacological methods, it has detrimental effects on women’s
reproductive health. The absence of research to date on the anti-vaginal inflammatory effects of Jirug-6 suppositories - a
traditional formulation derived from ancient medicinal recipes and long-used in traditional mongolian medicine has pro
vided the rationale for conducting this study.
Aim:Study of the effects of Jirug-6 suppositories on the vaginal inflammatory lesion model induced by Candida albicans.
Materials and Methods:To establish the vaginal inflammatory lesion model in experimental animals, the inoculum was
prepared according to the McFarland standard method, and 10 µL of the inoculum was intravaginally administered daily
for 4 consecutive days. Fifty-six mice with induced vaginal candidiasis were divided into 7 groups. On treatment days 1,
3, 5, 7, and 10, the vaginal cavity of the mice was washed with 100 µL of phosphate buffer. From the lavage fluid, 20 µL
was collected, mixed with 480 µL of phosphate buffer, and thoroughly homogenized. Using a 20 µL micropipette, three
aliquots were inoculated onto Sabouraud Dextrose Agar (SDA) medium and incubated at 37°C for 48 hours. Colonies
were counted, and at the end of the experiment, serum was isolated from blood samples, and IFN-γ and IL-10 levels in
the samples were quantified using ELISA. Histopathological analysis was performed on vaginal tissues from the experi
mental animals.
Results:In the experimental animals, Jirug-6 suppositories 0.4 mg/kg dose exhibited the mildest clinical signs of hyper
emia, swelling, hemorrhage, infiltration, or fluid loss into soft tissues in the vaginal and cervical regions (p<0.01). For
cervical inflammation, Jirug-6 suppositories 0.4 mg/kg demonstrated a strong anti-inflammatory effect with a score of
1.99±0.78, which was comparable to the positive control group (p<0.01). In serum, the concentrations of IFN-γ and IL-10
in Jirug-6 suppositories 0.4 mg/kg were determined to be 8.18±0.61 and 7.91±1.30, respectively, showing reduced levels
similar to those of the positive control group (p<0.01).
Conclusions:1. Jirug-6 suppositories reduce inflammatory symptoms and suppresses fungal dissemination during the vaginal lesion
model induced by C. albicans.
2. At a Jirug-6 suppositories 0.4 mg/kg demonstrates anti-inflammatory effects by increasing IFN-γ concentration and
decreasing IL-10 concentration in the C. albicans-induced vaginal lesion model. Furthermore, it enhances immunity
and reduces swelling during inflammation, exhibiting positive therapeutic effects against clinical symptoms.
- Full text:202505201905162401482-87.pdf
